TYW1：一种参与怀丁碱基生物合成的自由基S-腺苷甲硫氨酸酶。
TYW1: A Radical SAM Enzyme Involved in the Biosynthesis of Wybutosine Bases.
作者信息
Young Anthony P, Bandarian Vahe
机构信息
Department of Chemistry, University of Utah, Salt Lake City, UT, United States.
Department of Chemistry, University of Utah, Salt Lake City, UT, United States.
出版信息
Methods Enzymol. 2018;606:119-153. doi: 10.1016/bs.mie.2018.04.024. Epub 2018 Jun 6.
Abstract
Transfer RNA is extensively modified by the actions of a variety of enzymes. The radical S-adenosyl-l-methionine enzyme TYW1 modifies tRNA forming the characteristic tricyclic ring via the condensation of carbons 2 and 3 of pyruvate. This chapter details methods that are required for studies of TYW1.
摘要
转运RNA会受到多种酶的广泛修饰。自由基S-腺苷-L-甲硫氨酸酶TYW1通过丙酮酸的2号和3号碳原子缩合来修饰tRNA，形成特征性的三环结构。本章详细介绍了研究TYW1所需的方法。
相似文献
1
TYW1: A Radical SAM Enzyme Involved in the Biosynthesis of Wybutosine Bases.TYW1：一种参与怀丁碱基生物合成的自由基S-腺苷甲硫氨酸酶。
Methods Enzymol. 2018;606:119-153. doi: 10.1016/bs.mie.2018.04.024. Epub 2018 Jun 6.
2
Crystal structure of the radical SAM enzyme catalyzing tricyclic modified base formation in tRNA.催化tRNA中三环修饰碱基形成的自由基S-腺苷甲硫氨酸酶的晶体结构。
J Mol Biol. 2007 Oct 5;372(5):1204-14. doi: 10.1016/j.jmb.2007.07.024. Epub 2007 Jul 26.
3
Spectroscopic evidence for cofactor-substrate interaction in the radical-SAM enzyme TYW1.光谱证据表明自由基-SAM 酶 TYW1 中的辅因子-底物相互作用。
Dalton Trans. 2017 Oct 10;46(39):13211-13219. doi: 10.1039/c7dt00736a.
4
4-Demethylwyosine synthase from Pyrococcus abyssi is a radical-S-adenosyl-L-methionine enzyme with an additional [4Fe-4S](+2) cluster that interacts with the pyruvate co-substrate.来自 Pyrococcus abyssi 的 4-去甲基胞苷合成酶是一种依赖于 [4Fe-4S](+2) 簇的自由基-S-腺苷甲硫氨酸酶，该簇与丙酮酸辅酶相互作用。
J Biol Chem. 2012 Nov 30;287(49):41174-85. doi: 10.1074/jbc.M112.405019. Epub 2012 Oct 5.
5
Identification of the Radical SAM Enzymes Involved in the Biosynthesis of Methanopterin and Coenzyme F in Methanogens.产甲烷菌中参与蝶呤和辅酶F生物合成的自由基S-腺苷甲硫氨酸酶的鉴定
Methods Enzymol. 2018;606:461-483. doi: 10.1016/bs.mie.2018.04.015. Epub 2018 May 10.
6
Mechanistic Studies of the Radical S-Adenosyl-L-methionine Enzyme 4-Demethylwyosine Synthase Reveal the Site of Hydrogen Atom Abstraction.自由基S-腺苷-L-甲硫氨酸酶4-去甲基怀俄苷合成酶的机制研究揭示了氢原子提取位点。
Biochemistry. 2015 Jun 16;54(23):3569-72. doi: 10.1021/acs.biochem.5b00476. Epub 2015 Jun 8.
7
Biochemical and Structural Characterization of a Schiff Base in the Radical-Mediated Biosynthesis of 4-Demethylwyosine by TYW1.TYW1 介导的 4-去甲基胞苷生物合成中席夫碱的生化和结构特征
J Am Chem Soc. 2018 Jun 6;140(22):6842-6852. doi: 10.1021/jacs.8b01493. Epub 2018 May 24.
8
Insertion of 4-Demethylwyosine in tRNA Catalyzed by the Radical -Adenosyl-l-methionine Enzyme TYW1 Entails Oxidative Cleavage of Pyruvate to Form CO.TYW1 酶的 - 腺嘌呤基 -L- 蛋氨酸自由基催化的 tRNA 中的 4-去甲基假尿嘧啶插入涉及丙酮酸的氧化裂解形成 CO。
Biochemistry. 2022 Dec 6;61(23):2643-2647. doi: 10.1021/acs.biochem.2c00519. Epub 2022 Nov 3.
9
Structure of an archaeal TYW1, the enzyme catalyzing the second step of wye-base biosynthesis.古细菌TYW1的结构，该酶催化怀碱基生物合成的第二步。
Acta Crystallogr D Biol Crystallogr. 2007 Oct;63(Pt 10):1059-68. doi: 10.1107/S0907444907040668. Epub 2007 Sep 19.
10
Radical SAM enzymes involved in the biosynthesis of purine-based natural products.参与嘌呤类天然产物生物合成的自由基S-腺苷甲硫氨酸酶。
Biochim Biophys Acta. 2012 Nov;1824(11):1245-53. doi: 10.1016/j.bbapap.2012.07.014. Epub 2012 Aug 3.
引用本文的文献
1
Not all 5'-deoxyadenosines are created equal: Tracing the provenance of 5'-deoxyadenosine formed by the radical S-adenosyl-L-methionine enzyme 7-carboxy-7-deazaguanine synthase.并非所有的5'-脱氧腺苷都是一样的：追踪由自由基S-腺苷-L-甲硫氨酸酶7-羧基-7-脱氮鸟嘌呤合酶形成的5'-脱氧腺苷的来源。
J Biol Chem. 2025 Apr;301(4):108347. doi: 10.1016/j.jbc.2025.108347. Epub 2025 Feb 25.
2
Wybutosine hypomodification of tRNAphe activates HERVK and impairs neuronal differentiation.tRNAphe的怀丁苷酸低修饰激活内源性逆转录病毒K（HERVK）并损害神经元分化。
iScience. 2024 Apr 16;27(5):109748. doi: 10.1016/j.isci.2024.109748. eCollection 2024 May 17.
3
Anticodon stem-loop tRNA modifications influence codon decoding and frame maintenance during translation.反密码子茎环tRNA修饰在翻译过程中影响密码子解码和阅读框维持。
Semin Cell Dev Biol. 2024 Feb 15;154(Pt B):105-113. doi: 10.1016/j.semcdb.2023.06.003. Epub 2023 Jun 28.
4
Peptide Selenocysteine Substitutions Reveal Direct Substrate-Enzyme Interactions at Auxiliary Clusters in Radical -Adenosyl-l-methionine Maturases.肽硒代半胱氨酸取代揭示了在自由基 -腺苷基-l-甲硫氨酸甲基转移酶辅助簇中的直接底物-酶相互作用。
J Am Chem Soc. 2023 May 10;145(18):10167-10177. doi: 10.1021/jacs.3c00831. Epub 2023 Apr 27.
5
Insertion of 4-Demethylwyosine in tRNA Catalyzed by the Radical -Adenosyl-l-methionine Enzyme TYW1 Entails Oxidative Cleavage of Pyruvate to Form CO.TYW1 酶的 - 腺嘌呤基 -L- 蛋氨酸自由基催化的 tRNA 中的 4-去甲基假尿嘧啶插入涉及丙酮酸的氧化裂解形成 CO。
Biochemistry. 2022 Dec 6;61(23):2643-2647. doi: 10.1021/acs.biochem.2c00519. Epub 2022 Nov 3.
6
Site-Specific Profiling of 4-Thiouridine Across Transfer RNA Genes in .在……中对转运RNA基因上的4-硫尿苷进行位点特异性分析
ACS Omega. 2022 Jan 27;7(5):4011-4025. doi: 10.1021/acsomega.1c05071. eCollection 2022 Feb 8.
7
Eukaryotic TYW1 Is a Radical SAM Flavoenzyme.真核生物 TYW1 是一种依赖于自由基的 SAM 黄素酶。
Biochemistry. 2021 Jul 13;60(27):2179-2185. doi: 10.1021/acs.biochem.1c00349. Epub 2021 Jun 29.
8
In-depth analysis reveals complex molecular aetiology in a cohort of idiopathic cerebral palsy.深入分析揭示了特发性脑瘫患者队列中的复杂分子病因。
Brain. 2022 Mar 29;145(1):119-141. doi: 10.1093/brain/awab209.
9
Mechanistic Investigation of 1,2-Diol Dehydration of Paromamine Catalyzed by the Radical -Adenosyl-l-methionine Enzyme AprD4.自由基 - 腺苷甲硫氨酸酶 AprD4 催化的帕罗米胺 1,2-二醇脱水的机理研究。
J Am Chem Soc. 2021 Apr 7;143(13):5038-5043. doi: 10.1021/jacs.1c00076. Epub 2021 Mar 30.
10
Biochemical Pathways Leading to the Formation of Wyosine Derivatives in tRNA of Archaea.古菌 tRNA 中尿嘧啶衍生物形成的生化途径。
Biomolecules. 2020 Dec 2;10(12):1627. doi: 10.3390/biom10121627.
本文引用的文献
1
Spectroscopic evidence for cofactor-substrate interaction in the radical-SAM enzyme TYW1.光谱证据表明自由基-SAM 酶 TYW1 中的辅因子-底物相互作用。
Dalton Trans. 2017 Oct 10;46(39):13211-13219. doi: 10.1039/c7dt00736a.
2
Mechanistic Studies of the Radical S-Adenosyl-L-methionine Enzyme 4-Demethylwyosine Synthase Reveal the Site of Hydrogen Atom Abstraction.自由基S-腺苷-L-甲硫氨酸酶4-去甲基怀俄苷合成酶的机制研究揭示了氢原子提取位点。
Biochemistry. 2015 Jun 16;54(23):3569-72. doi: 10.1021/acs.biochem.5b00476. Epub 2015 Jun 8.
3
Radical SAM enzyme QueE defines a new minimal core fold and metal-dependent mechanism. radical SAM 酶 QueE 定义了一个新的最小核心折叠和金属依赖性机制。
Nat Chem Biol. 2014 Feb;10(2):106-12. doi: 10.1038/nchembio.1426. Epub 2013 Dec 22.
4
Menaquinone biosynthesis: formation of aminofutalosine requires a unique radical SAM enzyme.甲萘醌生物合成：支链氨基酸的形成需要一种独特的自由基 S-腺苷甲硫氨酸酶。
J Am Chem Soc. 2013 Oct 16;135(41):15318-21. doi: 10.1021/ja408594p. Epub 2013 Oct 7.
5
GenK-catalyzed C-6' methylation in the biosynthesis of gentamicin: isolation and characterization of a cobalamin-dependent radical SAM enzyme.GenK 催化庆大霉素生物合成中的 C-6' 甲基化：钴胺素依赖的自由基 SAM 酶的分离与表征。
J Am Chem Soc. 2013 Jun 5;135(22):8093-6. doi: 10.1021/ja312641f. Epub 2013 May 21.
6
MODOMICS: a database of RNA modification pathways--2013 update.MODOMICS：RNA 修饰途径数据库——2013 年更新版。
Nucleic Acids Res. 2013 Jan;41(Database issue):D262-7. doi: 10.1093/nar/gks1007. Epub 2012 Oct 30.
7
4-Demethylwyosine synthase from Pyrococcus abyssi is a radical-S-adenosyl-L-methionine enzyme with an additional [4Fe-4S](+2) cluster that interacts with the pyruvate co-substrate.来自 Pyrococcus abyssi 的 4-去甲基胞苷合成酶是一种依赖于 [4Fe-4S](+2) 簇的自由基-S-腺苷甲硫氨酸酶，该簇与丙酮酸辅酶相互作用。
J Biol Chem. 2012 Nov 30;287(49):41174-85. doi: 10.1074/jbc.M112.405019. Epub 2012 Oct 5.
8
Pyruvate is the source of the two carbons that are required for formation of the imidazoline ring of 4-demethylwyosine.丙酮酸是形成 4-去甲基胸苷中咪唑环所需的两个碳原子的来源。
Biochemistry. 2011 Dec 13;50(49):10573-5. doi: 10.1021/bi2015053. Epub 2011 Nov 14.
9
Discovery of epoxyqueuosine (oQ) reductase reveals parallels between halorespiration and tRNA modification.环氧假尿嘧啶核苷（oQ）还原酶的发现揭示了盐呼吸和 tRNA 修饰之间的相似性。
Proc Natl Acad Sci U S A. 2011 May 3;108(18):7368-72. doi: 10.1073/pnas.1018636108. Epub 2011 Apr 18.
10
Radical SAM activation of the B12-independent glycerol dehydratase results in formation of 5'-deoxy-5'-(methylthio)adenosine and not 5'-deoxyadenosine.B12 非依赖型甘油脱水酶的激进 SAM 激活导致形成 5'-脱氧-5'-(甲硫基)腺苷，而不是 5'-脱氧腺苷。
Biochemistry. 2011 Feb 1;50(4):440-2. doi: 10.1021/bi101255e. Epub 2011 Jan 5.
Zotero 插件