Li Heng, Cao Yuting, Li Shuai, Jiang Yun, Chen Jianqi, Wu Zhuofu
Computer Science Department, Information and Technology College, Jilin Agricultural University, Changchun 130118, PR China; Key Laboratory for Molecular Enzymology and Engineering of the Ministry of Education, College of Life Sciences, Jilin University, Changchun 130012, PR China.
Jilin Province Innovation Platform of Straw Comprehensive Utilization Technology College of Life Science, Jilin Agricultural University, Changchun 130118, PR China.
J Biosci Bioeng. 2019 Jan;127(1):38-44. doi: 10.1016/j.jbiosc.2018.07.009. Epub 2018 Aug 8.
In this study, an amino-functionalized silica matrix encapsulating β-galactosidase was first synthesized in situ, with subsequent covalent anchoring of lysozyme to the outer part of the amino-grafted matrix. Fourier transform infrared (FTIR) spectra verified that β-galactosidase was successfully encapsulated. Meanwhile, the co-immobilized enzymes were demonstrated to retain suitable enzymatic activities and outstanding operational stability during successive reaction cycles. Furthermore, when used for lactose removal from skim milk, the packed-bed column system achieved both a high lactose hydrolysis rate and microbial inactivation ratio during 30 days of continuous operation. Notably, this system exhibited favorable stability during 60 days of continuous hydrolysis of lactose in solution and thus may be appropriate for further development for use in industrial lactose removal from milk.
在本研究中，首先原位合成了一种包封β-半乳糖苷酶的氨基功能化二氧化硅基质，随后将溶菌酶共价锚定到氨基接枝基质的外部。傅里叶变换红外（FTIR）光谱证实β-半乳糖苷酶已成功包封。同时，在连续反应循环中，共固定化酶表现出保留了合适的酶活性和出色的操作稳定性。此外，当用于从脱脂牛奶中去除乳糖时，填充床柱系统在连续运行30天期间实现了高乳糖水解率和微生物灭活率。值得注意的是，该系统在溶液中乳糖连续水解60天期间表现出良好的稳定性，因此可能适合进一步开发用于从牛奶中工业去除乳糖。
