Laboratório de Biotecnologia de Alimentos, Universidade Do Vale Do Taquari - Univates, Av. Avelino Talini, Lajeado, RS, 171, ZC 95914-014, Brazil.
Programa de Pós-Graduação Em Biotecnologia, Universidade Do Vale Do Taquari - Univates, Lajeado, RS, Brazil.
Bioprocess Biosyst Eng. 2024 Feb;47(2):263-273. doi: 10.1007/s00449-023-02960-8. Epub 2023 Dec 29.
The objective of this study was to develop a bioprocess for lactose hydrolysis in diverse dairy matrices, specifically skim milk and cheese whey, utilizing column reactors employing a core-shell enzymatic system featuring β-galactosidase fused to a Cellulose Binding Domain (CBD) tag (β-galactosidase-CBD). The effectiveness of reactor configurations, including ball columns and toothed columns operating in packed and fluidized-bed modes, was evaluated for catalyzing lactose hydrolysis in both skim milk and cheese whey. In a closed system, these reactors achieved lactose hydrolysis rates of approximately 50% within 5 h under all evaluated conditions. Considering the scale of the bioprocess, the developed enzymatic system was capable of continuously hydrolyzing 9.6 L of skim milk while maintaining relative hydrolysis levels of approximately 50%. The biocatalyst, created by immobilizing β-galactosidase-CBD on magnetic core-shell capsules, exhibited exceptional operational stability, and the proposed bioprocess employing these column reactors showcases the potential for scalability.
本研究旨在开发一种用于不同乳制品基质（即脱脂乳和奶酪乳清）中乳糖水解的生物工艺，该工艺利用柱式反应器，采用β-半乳糖苷酶与纤维素结合结构域（CBD）标签融合的核壳酶系统（β-半乳糖苷酶-CBD）。评估了球柱和齿柱在填充床和流化床模式下的各种反应器构型在催化脱脂乳和奶酪乳清中乳糖水解方面的有效性。在封闭系统中，这些反应器在所有评估条件下，在 5 小时内可实现约 50%的乳糖水解率。考虑到生物工艺的规模，开发的酶系统能够连续水解 9.6 升脱脂乳，同时保持约 50%的相对水解水平。通过将β-半乳糖苷酶-CBD固定在磁性核壳胶囊上制备的生物催化剂表现出卓越的操作稳定性，并且使用这些柱式反应器的生物过程展示了可扩展性的潜力。
