Department of Microbiology, Soochow University, Taipei, Taiwan 111, Republic of China.
Department of Microbiology, Soochow University, Taipei, Taiwan 111, Republic of China.
Int J Food Microbiol. 2018 Dec 2;286:139-147. doi: 10.1016/j.ijfoodmicro.2018.08.008. Epub 2018 Aug 8.
Peracetic acid (PAA) is a common oxidative sanitizer that is used in the food industry against various microorganisms. Limited information on the response of bacteria to this biocide is available. This study investigates the molecular response of the prevalent seafood-borne pathogenic Vibrio parahaemolyticus to PAA using mutants of peroxide scavenging genes. Among katE1, katE2, katG1, katG2, ahpC1 and ahpC2, and their regulator oxyR gene mutants, oxyR and katE mutants were highly susceptible to PAA. The growth and lethality of V. parahaemolyticus were harmed by 15 ppm of PAA in the △katE1E2 double mutant, and were significantly ameliorated in the presence of the katE1 gene in the wild-type strain and the gene-complementary strains that were pre-adapted in 2 ppm of PAA or 100 μM hydrogen peroxide. The application of PAA to these strains induced the accumulation of reactive oxygen species. The reduction of the level of hydrogen peroxide and gene expression during this treatment was influenced by the presence of katE genes. This investigation confirmed the major role of katE1 and a compensatory role of katE2 in the resistance of V. parahaemolyticus to PAA, and demonstrated some minor differences in the responses of this bacterium against PAA and hydrogen peroxide.
过氧乙酸（PAA）是一种常见的氧化消毒剂，用于食品工业以对抗各种微生物。关于细菌对此种杀生物剂的反应的信息有限。本研究使用过氧化物清除基因的突变体来研究普遍存在的海鲜病原体副溶血性弧菌对 PAA 的分子反应。在 katE1、katE2、katG1、katG2、ahpC1 和 ahpC2 及其调节子 oxyR 基因突变体中，oxyR 和 katE 突变体对 PAA 高度敏感。在 △katE1E2 双突变体中，15ppm 的 PAA 损害了副溶血性弧菌的生长和致死性，而在野生型菌株和经过 2ppm PAA 或 100µM 过氧化氢预适应的基因互补菌株中，katE1 基因的存在显著改善了这种情况。PAA 的应用诱导了活性氧物质的积累。过氧化氢水平的降低和基因表达在这种处理过程中受到 katE 基因的影响。本研究证实了 katE1 在副溶血性弧菌对 PAA 的抗性中的主要作用和 katE2 的补偿作用，并表明该细菌对 PAA 和过氧化氢的反应存在一些细微差异。
