大鼠肾小球旁器简化灌流系统的研制。细胞松弛素B对自发性及促肾上腺皮质激素刺激的皮质类固醇生成的影响。

Development of a simplified perifusion system of rat zona glomerulosa. Effect of cytochalasin B on spontaneous and ACTH-stimulated corticosteroidogenesis.

作者信息

Feuilloley M, Netchitailo P, Lihrmann I, Delarue C, Vaudry H

出版信息

J Steroid Biochem. 1986 Jan;24(1):331-4. doi: 10.1016/0022-4731(86)90075-0.

Abstract

The aim of the present study was to develop a perifusion technique for rat adrenal glomerulosa slices as a model to study the kinetics of corticosteroid production in vitro. Perifusion of adrenal tissue with increasing concentration of ACTH (3.16 X 10(-12) to 10(-9) M) led to a dose-related stimulation of corticosterone and aldosterone secretion. Administration of cytochalasin B did not alter the basal secretion of corticosterone and aldosterone. In addition, cytochalasin B did not modify the response of glomerulosa tissue to ACTH. The results indicate that the perifusion model for glomerulosa fragments may provide valuable information, concerning the kinetics of steroid production and its regulation at the cellular level.

摘要

本研究的目的是开发一种用于大鼠肾上腺球状带切片的灌流技术，作为体外研究皮质类固醇生成动力学的模型。用浓度递增的促肾上腺皮质激素（3.16×10⁻¹²至10⁻⁹M）对肾上腺组织进行灌流，导致皮质酮和醛固酮分泌呈剂量相关的刺激。细胞松弛素B的给药并未改变皮质酮和醛固酮的基础分泌。此外，细胞松弛素B并未改变球状带组织对促肾上腺皮质激素的反应。结果表明，球状带碎片的灌流模型可能提供有关类固醇生成动力学及其在细胞水平调控的有价值信息。