正常和营养不良鸡的肌浆网的分离与特性研究

Isolation and characterization of sarcoplasmic reticulum from normal and dystrophic chicken.

作者信息

Kawamoto R M, Baskin R J

出版信息

Muscle Nerve. 1986 Mar-Apr;9(3):248-56. doi: 10.1002/mus.880090309.

DOI:10.1002/mus.880090309

Abstract

Purified sarcoplasmic reticulum vesicles from normal and dystrophic chicken skeletal muscle have been isolated by a procedure employing pressure disruption of a microsomal suspension. The dystrophic sarcoplasmic reticulum (SR) exhibited reduced Ca++ transport and phosphoenzyme formation, but the Ca++-ATPase activity was normal. Normal and dystrophic SR showed similar lipid profiles, except for a significant increase in free fatty acids in the dystrophic SR. Investigations involving the interaction of oleic acid with normal SR showed fatty acids can induce conditions similar to those found in dystrophic SR.

摘要

通过采用微粒体悬浮液压力破碎的方法，已从正常和营养不良的鸡骨骼肌中分离出纯化的肌浆网囊泡。营养不良的肌浆网（SR）表现出钙转运和磷酸酶形成减少，但钙ATP酶活性正常。正常和营养不良的SR显示出相似的脂质谱，除了营养不良的SR中游离脂肪酸显著增加。涉及油酸与正常SR相互作用的研究表明，脂肪酸可诱导出与营养不良的SR中发现的情况相似的条件。

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