School of Chemical Engineering, The University of Adelaide, Adelaide, Australia.
Environ Technol. 2020 Feb;41(6):703-710. doi: 10.1080/09593330.2018.1509887. Epub 2018 Aug 24.
Bactericidal proteins from the seed are reported to be suitable alternatives to conventional methods of bacterial reduction in water. In this study the cationic bactericidal proteins were isolated by attachment onto the surface of silicon dioxide. This functionalised SiO(ƒ-SiO) was then exposed to and to examine whether the ƒ-SiO could be used to inactivate the bacteria. The effect of the non-ionic surfactant dodecyl glucoside on the attachment of these bacteria to the ƒ-SiO was examined with the aim of developing a method of reusable bacterial inactivation. The primary result of this study was that the could be readily separated from the ƒ-SiO, allowing the ƒ-SiO to be used for further bacterial inactivation. The regeneration of the ƒ-SiO was demonstrated using fluorescence microscopy on bacterial cells stained with propidium iodide, and zeta potential measurements. Future applications of this work include a reusable method of removing bacteria from contaminated water.
种子中的杀菌蛋白被报道为可替代传统的水中细菌减少方法的合适选择。在这项研究中，通过附着在二氧化硅表面来分离阳离子杀菌蛋白。然后将这种功能化的 SiO(ƒ-SiO)暴露于 和 ，以检查 ƒ-SiO 是否可用于使细菌失活。研究了非离子表面活性剂十二烷基葡萄糖苷对这些细菌附着到 ƒ-SiO 的影响，目的是开发一种可重复使用的细菌失活方法。这项研究的主要结果是，可以很容易地将 从 ƒ-SiO 中分离出来，从而可以将 ƒ-SiO 用于进一步的细菌失活。通过使用碘化丙啶染色的细菌细胞的荧光显微镜和 ζ 电位测量来证明 ƒ-SiO 的再生。这项工作的未来应用包括一种从污染水中去除细菌的可重复使用的方法。
