Center for Neural Science, New York University, New York, New York.
Neuroscience Institute, Langone Medical Center, New York University, New York, New York.
Int J Eat Disord. 2018 Aug;51(8):1020-1025. doi: 10.1002/eat.22937. Epub 2018 Aug 13.
This study tested the effects of ketamine on vulnerability of female adolescent mice to activity-based anorexia (ABA).
Twenty-four female C57Bl/6 J mice underwent ABA induction, which involved exposing wheel-acclimated adolescent mice to two bouts of food restriction (FR)-the first ABA (P41-44, mid-adolescence) and the second ABA (P55-59, late adolescence), with recovery in between. Ketamine (3 or 30 mg/kg) or vehicle was given once, on the second day of FR of the first ABA (P42). Food consumption, body weight and wheel running activity were measured daily. Anxiety-like behaviors were accessed by elevated plus maze on P49 and P62, after weight restoration during the recovery phase.
Ketamine (30 mg/kg) increased food intake during the first ABA (+38%, p = .015) and facilitated weight gain during recovery (+42%, p = .003). During the second ABA, the effect was manifested as increased food intake (+38%, p = .001) and weight gain (+47%, p = .001) while attenuating FR-induced wheel running activity (-24%, p = .09) and weight loss (-17%, p = .056). Ketamine also reduced anxiety-like behaviors.
Thus, single injection of ketamine during mid-adolescence effectively attenuates vulnerability of female mice to repeated ABA exposures.
本研究测试了氯胺酮对雌性青春期小鼠易患基于活动的厌食症（ABA）的影响。
24 只 C57Bl/6 J 雌性小鼠接受 ABA 诱导，包括使适应轮的青春期小鼠经历两次食物限制（FR）-第一次 ABA（P41-44，青春期中期）和第二次 ABA（P55-59，青春期后期），中间有恢复期。氯胺酮（3 或 30mg/kg）或载体在第一次 ABA 的 FR 的第二天（P42）给予一次。每天测量食物消耗、体重和轮跑活动。在恢复期间体重恢复后，在 P49 和 P62 通过高架十字迷宫测量焦虑样行为。
氯胺酮（30mg/kg）增加了第一次 ABA 期间的食物摄入量（增加 38%，p=0.015）并促进了恢复期间的体重增加（增加 42%，p=0.003）。在第二次 ABA 中，这种作用表现为增加食物摄入量（增加 38%，p=0.001）和体重增加（增加 47%，p=0.001），同时减轻 FR 诱导的轮跑活动减少（减少 24%，p=0.09）和体重减轻（减少 17%，p=0.056）。氯胺酮还降低了焦虑样行为。
因此，青春期中期单次注射氯胺酮可有效减轻雌性小鼠对重复 ABA 暴露的易感性。
