Department of Biology and Environmental Sciences, University of Gothenburg, Gothenburg, SE-405 30, Göteborg, Sweden.
Mol Plant Pathol. 2019 Jan;20(1):69-77. doi: 10.1111/mpp.12740. Epub 2018 Oct 11.
Non-specific lipid transfer proteins (LTPs) are involved in the transport of lipophilic compounds to the cuticular surface in epidermal cells and in the defence against pathogens. The role of glycophosphatidylinositol (GPI)-anchored LTPs (LTPGs) in resistance against non-host mildews in Arabidopsis thaliana was investigated using reverse genetics. Loss of either LTPG1, LTPG2, LTPG5 or LTPG6 increased the susceptibility to penetration of the epidermal cell wall by Blumeria graminis f. sp. hordei (Bgh). However, no impact on pre-penetration defence against another non-host mildew, Erysiphe pisi (Ep), was observed. LTPG1 was localized to papillae at the sites of Bgh penetration. This study shows that, in addition to the previously known functions, LTPGs contribute to pre-invasive defence against certain non-host powdery mildew pathogens.
非特异性脂质转移蛋白（LTPs）参与亲脂性化合物向表皮细胞角质层表面的运输，并参与对病原体的防御。本研究使用反向遗传学方法研究了糖基磷脂酰肌醇（GPI）锚定的 LTP（LTPG）在拟南芥中对非寄主霉菌的抗性中的作用。LTPG1、LTPG2、LTPG5 或 LTPG6 的缺失均增加了表皮细胞壁被禾谷布氏白粉菌（Bgh）穿透的敏感性。然而，对另一种非寄主霉菌豌豆白粉菌（Ep）的预穿透防御没有影响。LTPG1 定位于 Bgh 穿透部位的乳突中。本研究表明，除了先前已知的功能外，LTPG 还有助于对某些非寄主粉霉病原体的侵入前防御。
