δ9-四氢大麻酚对小鼠巨噬细胞体外吞噬作用的抑制效应

Suppressive effect of delta 9-tetrahydrocannabinol in vitro on phagocytosis by murine macrophages.

作者信息

Friedman M, Cepero M L, Klein T, Friedman H

出版信息

Proc Soc Exp Biol Med. 1986 Jun;182(2):225-8. doi: 10.3181/00379727-182-42332.

Abstract

Incubation of normal mouse peritoneal cells consisting of over 90% phagocytizing macrophages with delta 9-tetrahydrocannabinol (THC) resulted in a inhibition of phagocytic function. The THC in a dose-related manner suppressed the percentage of macrophages per culture which ingested yeast and the average number of yeast particles ingested by the phagocytizing macrophages. The vehicle used to suspend the THC in vitro, i.e., DMSO, had no detectable effect on macrophage function. Suppression of phagocytosis with no effects on viability or cell number occurred with doses of 10 micrograms or less THC per milliliter culture medium. Measurable suppression also occurred after 24- to 48-hr treatment of the macrophages with the THC. This compound had little if any detectable effect on phagocytosis when added directly to the cultures shortly before testing for phagocytosis. Further studies concerning the effects of THC on macrophage function appear warranted.

摘要

由超过90%具有吞噬作用的巨噬细胞组成的正常小鼠腹膜细胞与δ9-四氢大麻酚（THC）一起孵育，导致吞噬功能受到抑制。THC以剂量相关的方式抑制了每批培养物中摄取酵母的巨噬细胞百分比以及吞噬巨噬细胞摄取的酵母颗粒的平均数量。用于在体外悬浮THC的载体，即二甲基亚砜（DMSO），对巨噬细胞功能没有可检测到的影响。每毫升培养基中THC剂量为10微克或更低时，吞噬作用受到抑制，但对活力或细胞数量没有影响。用THC对巨噬细胞进行24至48小时处理后，也出现了可测量的抑制作用。在即将测试吞噬作用前不久直接将该化合物添加到培养物中时，它对吞噬作用几乎没有可检测到的影响。关于THC对巨噬细胞功能影响的进一步研究似乎是必要的。