Oswald Marvin Siegfried, Hansmann Martin-Leo
Universitätsklinikum Frankfurt/Main, Dr. Senckenberg Institut für Pathologie, Theodor-Stern-Kai 7, Frankfurt/Main, 60590, Hessen, Germany.
Universitätsklinikum Frankfurt/Main, Dr. Senckenberg Institut für Pathologie, Theodor-Stern-Kai 7, Frankfurt/Main, 60590, Hessen, Germany; Johann Wolfgang Goethe-Universität Frankfurt am Main, Frankfurt Institute for Advanced Studies (FIAS), Ruth-Moufang-Straße 1, Frankfurt/Main, 60438, Hessen, Germany.
Acta Histochem. 2018 Nov;120(8):720-727. doi: 10.1016/j.acthis.2018.08.001. Epub 2018 Aug 10.
Lymph node diagnostics are essentially based on cutting thin sections of formalin fixed tissues. After hematoxylin and eosin stain, Giemsa stain and immunohistochemical staining of these tissues, the lymph node diagnosis is done using a light microscope, looking at two-dimensional pictures. Three-dimensional visualizations of lymph node tissue have not been used in lymphoma diagnostics yet. This article describes three-dimensional visualization of lymphoid tissue, using thick paraffin sections, immunostained with monoclonal antibodies, confocal laser scanning and data processing with appropriate software and the 3D printing process itself. The advantages and disadvantages of different printing techniques are discussed as well as the application of 3D models in diagnostics, teaching and research of lymph nodes.
淋巴结诊断基本上基于对福尔马林固定组织进行薄切片。对这些组织进行苏木精和伊红染色、吉姆萨染色及免疫组化染色后，使用光学显微镜观察二维图像来进行淋巴结诊断。淋巴结组织的三维可视化尚未用于淋巴瘤诊断。本文描述了使用厚石蜡切片、用单克隆抗体进行免疫染色、共聚焦激光扫描以及使用适当软件进行数据处理和3D打印过程本身来实现淋巴组织的三维可视化。文中讨论了不同打印技术的优缺点以及3D模型在淋巴结诊断、教学和研究中的应用。
