Department of Biological Sciences, Seoul National University, Seoul, South Korea.
Department of Biological Sciences, Seoul National University, Seoul, South Korea
Mol Cell Biol. 2018 Oct 15;38(21). doi: 10.1128/MCB.00217-18. Print 2018 Nov 1.
The involvement of cell division in cellular differentiation has long been accepted. Cell division may be required not only for the expansion of a differentiated cell population but also for the execution of differentiation processes. Nonetheless, knowledge regarding how specific differentiation processes are controlled in a cell division-dependent manner is far from complete. Here, we determined the involvement of cell division in neuronal differentiation. We initially confirmed that cell division is an essential event for the neuronal differentiation of P19 embryonic carcinoma cells. We investigated the induction mechanisms of , whose expression is induced by retinoic acid (RA) in a cell division-dependent manner. Promoter analysis of revealed a specific region required for RA-dependent transcription. A series of experiments was used to identify E2F1 as the induction factor for the RA-dependent transcription of We propose that E2F1 mediates neuronal differentiation in a cell division-dependent manner.
细胞分裂参与细胞分化早已被人们所接受。细胞分裂不仅可能是分化细胞群体扩增所必需的，而且也可能是分化过程执行所必需的。尽管如此，关于特定分化过程如何以细胞分裂依赖性方式被控制的知识还远不完整。在这里，我们确定了细胞分裂在神经元分化中的参与。我们最初证实细胞分裂是 P19 胚胎癌细胞神经元分化所必需的事件。我们研究了 的诱导机制， 的表达在细胞分裂依赖性方式下被维甲酸（RA）诱导。对 的启动子分析揭示了 RA 依赖性转录所需的特定区域。一系列实验被用来鉴定 E2F1 是 的 RA 依赖性转录的诱导因子。我们提出 E2F1 以细胞分裂依赖性方式介导神经元分化。
