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Nkd2 促进牙囊干细胞/祖细胞向成骨细胞分化。

Nkd2 promotes the differentiation of dental follicle stem/progenitor cells into osteoblasts.

机构信息

Department of Stomatology, Shenzhen Children's Hospital, Shenzhen, Guangdong 518038, P.R. China.

Department of Operative Dentistry and Endodontics, Xiangya School of Stomatology, Xiangya Stomatological Hospital, Central South University, Changsha, Hu'nan 410083, P.R. China.

出版信息

Int J Mol Med. 2018 Nov;42(5):2403-2414. doi: 10.3892/ijmm.2018.3822. Epub 2018 Aug 14.

DOI:10.3892/ijmm.2018.3822
PMID:30106129
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6192769/
Abstract

Dental follicle stem/progenitor cells have the potential to undergo osteogenesis. naked cuticle homolog 2 (Nkd2) is a signal‑inducible feedback antagonist of the canonical Wnt signaling pathway. The purpose of the present study was to investigate the function of Nkd2 in the differentiation of dental follicle stem/progenitor cells (DFSCs) into osteoblasts. Immunohistochemistry, reverse transcription‑quantitative polymerase chain reaction and western blotting were employed to detect Nkd2 expression in rat DFSCs. In addition, rat DFSCs (rDFSCs) were transfected with small interfering RNAs to examine the effect of Nkd2 on the differentiation of these cells into osteoblasts. Furthermore, the function of Nkd2 in the Wnt/β‑catenin pathway in rDFSCs was investigated using β‑catenin/T‑cell factor luciferase activity assays and western blotting. It was revealed that the expression of Nkd2 was upregulated during the differentiation of rDFSCs into osteoblasts. Furthermore, osteoblast differentiation ability and Wnt/β‑catenin pathway activity were significantly decreased in Nkd2‑silenced rDFSCs compared with the si‑NC group (P<0.05 and P<0.001, respectively). The results suggest that Nkd2 promotes the differentiation of rDFSCs into osteoblasts through Wnt/β‑catenin signaling.

摘要

牙囊干细胞/祖细胞具有成骨潜能。裸皮同源物 2（Nkd2）是经典 Wnt 信号通路的信号诱导反馈拮抗剂。本研究旨在探讨 Nkd2 在牙囊干细胞/祖细胞（DFSCs）向成骨细胞分化中的作用。采用免疫组织化学、逆转录-定量聚合酶链反应和 Western blot 检测 Nkd2 在大鼠 DFSCs 中的表达。此外，用小干扰 RNA 转染大鼠 DFSCs（rDFSCs），以研究 Nkd2 对这些细胞向成骨细胞分化的影响。进一步通过β-连环蛋白/TCF 荧光素酶活性测定和 Western blot 检测 Nkd2 在 rDFSCs 中的 Wnt/β-连环蛋白通路中的功能。结果表明，rDFSCs 向成骨细胞分化过程中 Nkd2 的表达上调。与 si-NC 组相比，Nkd2 沉默的 rDFSCs 的成骨细胞分化能力和 Wnt/β-连环蛋白通路活性显著降低（P<0.05 和 P<0.001）。结果提示，Nkd2 通过 Wnt/β-连环蛋白信号促进 rDFSCs 向成骨细胞分化。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb32/6192769/ed11e68a2069/IJMM-42-05-2403-g05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb32/6192769/c0f95c33c0f0/IJMM-42-05-2403-g00.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb32/6192769/09d1d9778e8c/IJMM-42-05-2403-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb32/6192769/8ec5f4abb06f/IJMM-42-05-2403-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb32/6192769/7ebde6df3dea/IJMM-42-05-2403-g03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb32/6192769/cafc8671ef6d/IJMM-42-05-2403-g04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb32/6192769/ed11e68a2069/IJMM-42-05-2403-g05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb32/6192769/c0f95c33c0f0/IJMM-42-05-2403-g00.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb32/6192769/09d1d9778e8c/IJMM-42-05-2403-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb32/6192769/8ec5f4abb06f/IJMM-42-05-2403-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb32/6192769/7ebde6df3dea/IJMM-42-05-2403-g03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb32/6192769/cafc8671ef6d/IJMM-42-05-2403-g04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb32/6192769/ed11e68a2069/IJMM-42-05-2403-g05.jpg

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