College of Food Science and Engineering, Northwest A & F University, Yangling, Shaanxi, 712100, China; Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business University(BTBU), Beijing, 100048, China.
College of Food Science and Engineering, Northwest A & F University, Yangling, Shaanxi, 712100, China.
Chem Biol Interact. 2018 Dec 25;296:9-17. doi: 10.1016/j.cbi.2018.08.005. Epub 2018 Aug 11.
Apigenin (API) is a kind of important flavonoid present in temperate and tropical fruit and vegetables, especially the celery. It exerts anticancer, anti-bacterial, anti-viral, anti-inflammatory, anti-oxidation properties. In the present study, the mechanism of protective action of apigenin on 3-chloro-1, 2-propanediol (3-MCPD)-induced renal injury was investigated in rat. Sprague-Dawley (SD) rats were divided into five groups: control group (ultrapure water treated), CMC group (sodium carboxymethylcellulose treated), 3-MCPD treatment group (30 mg/kg body weight/day), 3-MCPD plus API co-treatment group (20, 40 mg/kg body weight/day). The results showed that API significantly reduced renal function markers, serum creatinine and urea nitrogen content. Besides, the renal tissue lesion in 3-MCPD treatment group was restored by API to some extent. We indicated that API exerted renoprotective effect by modulating oxidative phosphorylation especially up-regulated the expressions of ATP6 and ATP8, re-establishing mitochondrial membrane potential (MMP), relieving the increase of Bax/Bcl2 ratio, reducing cytochrome c release, thus inhibiting the activation of Caspase 9 and Caspase 3. In conclusion, apigenin possesses excellent protective effect against 3-MCPD-induced renal injury by modulating mitochondria dependent Caspase cascade pathway.
芹菜素（API）是一种存在于温带和热带水果和蔬菜中的重要类黄酮，尤其是芹菜。它具有抗癌、抗菌、抗病毒、抗炎、抗氧化作用。本研究旨在探讨芹菜素对 3-氯-1,2-丙二醇（3-MCPD）诱导的肾损伤的保护作用机制。SD 大鼠分为五组：对照组（给予超纯水）、CMC 组（给予羧甲基纤维素钠）、3-MCPD 处理组（30mg/kg 体重/天）、3-MCPD+API 共处理组（20、40mg/kg 体重/天）。结果表明，API 显著降低了肾功能标志物血清肌酐和尿素氮的含量。此外，API 部分恢复了 3-MCPD 处理组的肾脏组织损伤。我们表明，API 通过调节氧化磷酸化发挥肾保护作用，特别是上调 ATP6 和 ATP8 的表达，重建线粒体膜电位（MMP），减轻 Bax/Bcl2 比值的增加，减少细胞色素 c 的释放，从而抑制 Caspase 9 和 Caspase 3 的激活。总之，芹菜素通过调节线粒体依赖性 Caspase 级联途径对 3-MCPD 诱导的肾损伤具有良好的保护作用。
