Department of Advanced Free Radical Science, Tohoku University Graduate School of Dentistry, 4-1 Seiryo-machi, Aoba-ku, Sendai 980-8575, Japan.
Department of Advanced Free Radical Science, Tohoku University Graduate School of Dentistry, 4-1 Seiryo-machi, Aoba-ku, Sendai 980-8575, Japan.
J Photochem Photobiol B. 2018 Oct;187:96-105. doi: 10.1016/j.jphotobiol.2018.08.006. Epub 2018 Aug 7.
Antimicrobial chemotherapy based on ultraviolet-A (UVA) irradiation of polyphenol solution has been proposed as an adjunctive treatment for dental caries. However, the safety of this treatment has not been thoroughly evaluated. Therefore, the aim of the present study was to assess the influence of this treatment on the oral mucosa in hamsters and wounded skin in rats. An oral mucosal irritation test was performed in hamsters. The cheek pouch was everted and treated with UVA irradiation (wavelength: 365 nm) of pure water, 1 mg/mL of caffeic acid, or 1 mg/mL of grape seed extract using a light-emitting diode at an irradiance of 275 mW/cm. Each treatment was performed for 2 or 5 min and repeated three times. Macroscopic and histological evaluations were performed 24 h after the last treatment. We also examined the effects of the treatment on the healing process of skin wounds in rats. Full-thickness skin wounds created on the back of the rats were treated for 2 min as described above, but only once. The wound area was then assessed daily for 9 days. The results demonstrated that the treatment induced oral mucosal irritation depending on the irradiation time; however, it did not influence the wound healing process. The oral mucosal irritation potential of three treatment sessions performed for 2 and 5 min was minimal and mild to moderate, respectively, according to histological analysis. These findings suggest that the duration of treatment based on UVA irradiation of polyphenols in the oral cavity should be as short as possible, considering the clinical efficacy of the antimicrobial effects and the irritation potential.
基于多酚溶液的紫外线 A（UVA）照射的抗菌化疗已被提议作为龋齿的辅助治疗方法。然而，这种治疗方法的安全性尚未得到彻底评估。因此，本研究旨在评估这种治疗方法对仓鼠口腔黏膜和大鼠创伤皮肤的影响。在仓鼠中进行了口腔黏膜刺激试验。将颊囊外翻并用发光二极管以 275 mW/cm 的辐照度对纯水、1 mg/mL 咖啡酸或 1 mg/mL 葡萄籽提取物进行 UVA 照射（波长：365nm）进行处理。每种处理重复进行 2 或 5 分钟，共进行 3 次。最后一次处理后 24 小时进行宏观和组织学评估。我们还研究了该治疗方法对大鼠皮肤伤口愈合过程的影响。在大鼠背部创建全层皮肤伤口，如上所述处理 2 分钟，但仅处理一次。然后每天评估伤口面积 9 天。结果表明，治疗会根据辐照时间引起口腔黏膜刺激；然而，它不会影响伤口愈合过程。根据组织学分析，进行 2 分钟和 5 分钟的三个疗程的口腔黏膜刺激潜力分别为最小和轻度至中度。这些发现表明，考虑到抗菌效果和刺激潜力的临床疗效，口腔内基于多酚的 UVA 照射的治疗时间应尽可能短。
