一种从胚胎成纤维细胞发育而来的、表达药物诱导型小鼠多能性因子的裸鼹鼠诱导多能干细胞系。

A naked mole rat iPSC line expressing drug-inducible mouse pluripotency factors developed from embryonic fibroblasts.

作者信息

Lee Sang-Goo, Mikhalchenko Aleksei E, Yim Sun Hee, Gladyshev Vadim N

机构信息

Division of Genetics, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.

Division of Genetics, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA; Center for Data-Intensive Biomedicine and Biotechnology, Skolkovo Institute of Science and Technology, Moscow 143028, Russia.

出版信息

Stem Cell Res. 2018 Aug;31:197-200. doi: 10.1016/j.scr.2018.06.010. Epub 2018 Jun 20.

DOI:10.1016/j.scr.2018.06.010

PMID:30107334

Abstract

Naked mole rats (NMRs, Heterocephalus glaber) are long-lived, cancer-resistant rodents. Here, we report the development of an induced pluripotent stem cell (iPSC) line generated from immortalized NMR embryonic fibroblasts transduced with a doxycycline-inducible mouse OSKM polycistronic vector. This iPSC line was shown to express pluripotency-associated markers, form embryoid bodies, differentiate in vitro to the derivatives of three germ layers, and exhibit normal karyotype. The ability of iPSCs to differentiate in vivo was supported by the contribution to interspecific chimera upon injection into mouse blastocysts. This NMR iPSC line may be a useful tool in cancer and aging research.

摘要

裸鼹鼠（NMRs，Heterocephalus glaber）是寿命长且具有抗癌能力的啮齿动物。在此，我们报告了一种诱导多能干细胞（iPSC）系的建立，该细胞系由用强力霉素诱导型小鼠OSKM多顺反子载体转导的永生化裸鼹鼠胚胎成纤维细胞产生。该iPSC系被证明可表达多能性相关标志物，形成胚状体，在体外分化为三个胚层的衍生物，并具有正常的核型。将其注射到小鼠囊胚中对种间嵌合体有贡献，这支持了iPSC在体内分化的能力。这个裸鼹鼠iPSC系可能是癌症和衰老研究中的一个有用工具。