Wu H J, Gao H, Xie Y N, Zhang Y Y, Yang Z B, Zhang X M
College of Life Science, North China University of Science and Technology, Tangshan 063000, China.
Zhonghua Yu Fang Yi Xue Za Zhi. 2018 Aug 6;52(8):822-826. doi: 10.3760/cma.j.issn.0253-9624.2018.08.009.
This study aimed to investigate the relationship between the genetic variation of CD55 promoter and the risk of esophageal cancer. A total of 700 esophageal cancer patients recruited between April 2008 and December 2012 at Tangshan Grongren Hospital and Tangshan Renmin Hospital, and 700 frequency matched controls were randomly selected from a pool of cancer free subjects recruited from a nutritional survey. Genotypes of CD55 rs2564978 polymorphism among all subjects were conducted by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The and 95 were calculated by non-conditional Logistic regression to evaluate the association of CD55 rs2564978T/C polymorphism with the risk of esophageal cancer. The average age of cases and control was (60.04±9.19) and (59.21±9.98) years old. Compared with CD55 rs2564978 TT carriers, the individuals with CC genotype had a significantly higher risk of esophageal cancer (1.94, 95:1.42-2.66) . When stratified by sex, this genetic variation affected the risk of esophageal cancer among both males (1.92, 95:1.37-2.70) and females (2.34, 95:1.04-5.27). When stratified by age, the CD55 rs2564978 CC was associated with the susceptibility of developing esophageal cancer among younger individuals (1.79, 95:1.19-2.68) and older people (2.32, 95:1.41-3.83).When stratified by drinking status, CC genotype carriers increase the risk of esophageal cancer when drinking (1.93, 95:1.03-3.63) or not drinking (1.95, 95:1.36-2.80). When stratified by smoking status, CC genotype was associated with the risk of esophageal cancer among non-smokers (1.79, 95: 1.13-2.83), light smokers (less than 30 packs/year, 1.86, 95:1.31-2.64) and heavy smokers (more than 30 packs/year, 2.67, 95:1.28-5.57). Gene-environmental interaction analysis showed that CD55 rs2564978T/C polymorphism interacted with smoking status to increase the risk of esophageal cancer. CD55 rs2564978 polymorphism effects on the risk of esophageal cancer.
本研究旨在探讨CD55启动子的基因变异与食管癌风险之间的关系。2008年4月至2012年12月期间，在唐山工人医院和唐山人民医院招募了700例食管癌患者，并从营养调查中招募的无癌受试者中随机选取700例频率匹配的对照。采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法对所有受试者的CD55 rs2564978多态性进行基因分型。通过非条件Logistic回归计算比值比（OR）和95%可信区间（CI），以评估CD55 rs2564978T/C多态性与食管癌风险的关联。病例组和对照组的平均年龄分别为（60.04±9.19）岁和（59.21±9.98）岁。与CD55 rs2564978 TT携带者相比，CC基因型个体患食管癌的风险显著更高（OR=1.94，95%CI：1.42-2.66）。按性别分层时，这种基因变异影响男性（OR=1.92，95%CI：1.37-2.70）和女性（OR=2.34，95%CI：1.04-5.27）患食管癌的风险。按年龄分层时，CD55 rs2564978 CC与年轻个体（OR=1.79，95%CI：1.19-2.68）和老年人（OR=2.32，95%CI：1.41-3.83）患食管癌的易感性相关。按饮酒状况分层时，CC基因型携带者在饮酒（OR=1.93，95%CI：1.03-3.63）或不饮酒（OR=1.95，95%CI：1.36-2.80）时患食管癌的风险均增加。按吸烟状况分层时，CC基因型与非吸烟者（OR=1.79，95%CI：1.13-2.83）、轻度吸烟者（每年少于30包，OR=1.86，95%CI：1.31-2.64）和重度吸烟者（每年超过30包，OR=2.67，95%CI：1.28-5.57）患食管癌的风险相关。基因-环境交互作用分析表明，CD55 rs2564978T/C多态性与吸烟状况相互作用增加食管癌风险。CD55 rs2564978多态性对食管癌风险有影响。
