实验室规模下脂肪酶催化合成结构化脂质

Lipase-Catalyzed Synthesis of Structured Lipids at Laboratory Scale.

作者信息

Ferreira-Dias Suzana, Osório Natália M, Tecelão Carla

机构信息

Instituto Superior de Agronomia, Universidade de Lisboa, LEAF, Linking Landscape, Environment, Agriculture and Food, Lisbon, Portugal.

Escola Superior de Tecnologia do Barreiro, Instituto Politécnico de Setúbal, Barreiro, Portugal.

出版信息

Methods Mol Biol. 2018;1835:315-336. doi: 10.1007/978-1-4939-8672-9_17.

DOI:10.1007/978-1-4939-8672-9_17

PMID:30109660

Abstract

In this chapter, some examples of laboratory protocols to produce functional structured lipids, namely, human milk fat substitutes, dietetic triacylglycerols, and interesterified fat blends with improved nutritional and rheological properties, catalyzed either by immobilized commercial or noncommercial lipase preparations, are presented. In addition to batch synthesis, the continuous production in packed- or fluidized-bed bioreactors is addressed, as well as the evaluation of operational stability of the biocatalysts used (either in batch reuses or in continuous mode).

摘要

在本章中，将介绍一些实验室方案的实例，这些方案用于生产功能性结构脂质，即人乳脂肪替代品、营养性三酰甘油以及具有改善的营养和流变学特性的酯交换脂肪混合物，它们由固定化的商业或非商业脂肪酶制剂催化。除了间歇合成外，还讨论了在填充床或流化床生物反应器中的连续生产，以及所使用生物催化剂的操作稳定性评估（无论是间歇重复使用还是连续模式）。