Tian Lian-Chao, Qin Shao-Rong, Yi Hong, Li Chun, Ma Hui-Wen, Ren Cheng-Tao, Li Yun, Liu Xiao-Qian, Wang Zhi-Min
School of Traditional Chinese Medicine， Guangdong Pharmaceutical University， Guangzhou 510006, China.
National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicines, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences， Beijing 100700, China.
Zhongguo Zhong Yao Za Zhi. 2018 Jul;43(14):2850-2856. doi: 10.19540/j.cnki.cjcmm.20180514.009.
To establish a high performance liquid chromatography (HPLC) method for simultaneous determination of four alkaloids(arecoline， guvacoline， arecaidine， and guvacine) in Arecae Pericarpium (AP) and Arecae Semen (AS), and compare the contents of these four alkaloids between different medicinal parts. The chromatographic conditions were as follows:Welch SCX（4.6 mm×250 mm， 5 μm）column, with acetonitrile-0.2% phosphoric acid solution (adjusted to pH 3.85-3.90 with ammonium hydroxide) at 50:50 as the mobile phase, at a flow rate of 0.5 mL·min⁻¹. The column temperature was set at 35 °C, and the detection wavelength was 215 nm. The results of content determination in 7 batches of AS and 10 batches of AP showed that, the contents of 4 alkaloids in AS (arecaidine 0.020%-0.045%, guvacine 0.031%-0.086%, arecoline 0.194%-0.346%, and guvacoline 0.065%-0.094%) were generally higher than those in AP (arecaidine 0.10%-0.032%, guvacine 0.006%-0.029% arecoline 0.00%-0.070%, and guvacoline 0.00%-0.020%), and most of the APs had no arecoline and arecaidine at all in fruit peel. The above results indicated that different alkaloids can be used to distinguish the different medicinal parts of Arera catechu. Arecoline， guvacoline， arecaidine， and guvacine can be used as the quality control markers of AS, while for AP, only arecaidine and guvacine were needed.
建立高效液相色谱（HPLC）法同时测定槟榔壳（AP）和槟榔（AS）中4种生物碱（槟榔碱、去甲槟榔碱、槟榔次碱和去甲槟榔次碱）的含量，并比较不同药用部位这4种生物碱的含量。色谱条件如下：Welch SCX（4.6 mm×250 mm，5 μm）色谱柱，以乙腈 - 0.2%磷酸溶液（用氢氧化铵调节pH至3.85 - 3.90）按50:50作为流动相，流速为0.5 mL·min⁻¹。柱温设定为35℃，检测波长为215 nm。7批AS和10批AP的含量测定结果表明，AS中4种生物碱的含量（槟榔次碱0.020% - 0.045%，去甲槟榔次碱0.031% - 0.086%，槟榔碱0.194% - 0.346%，去甲槟榔碱0.065% - 0.094%）普遍高于AP（槟榔次碱0.10% - 0.032%，去甲槟榔次碱0.006% - 0.029%，槟榔碱0.00% - 0.070%，去甲槟榔碱0.00% - 0.020%），且多数AP的果皮中完全不含槟榔碱和槟榔次碱。上述结果表明，不同生物碱可用于区分槟榔的不同药用部位。槟榔碱、去甲槟榔碱、槟榔次碱和去甲槟榔次碱可作为AS的质量控制指标，而对于AP，仅需槟榔次碱和去甲槟榔次碱。
