Tocher D R, Sargent J R
Biochim Biophys Acta. 1986 May 21;876(3):592-600. doi: 10.1016/0005-2760(86)90048-2.
The incorporation of [1-14C]arachidonic acid and [1-14C]eicosapentaenoic acids into phospholipids was studied in peripheral blood neutrophils from plaice, a marine fish whose lipids are rich in (n-3) polyunsaturated fatty acids. The incorporation of both labelled fatty acids into phospholipids was approximately equal when presented individually, and at equal concentration, to the cells. Arachidonic acid was relatively preferred when both acids were present in the incubations. When incorporation was expressed per unit mass of phospholipid class, the order of incorporation was PI greater than PC greater than PE greater than PS greater than sphingomyelin for both fatty acids. However, the specificity for incorporation into PI was significantly greater with arachidonic acid. Eicosapentaenoic acid was incorporated into PC to a greater extent than arachidonic acid. The results are discussed in relation to the possible roles of arachidonic acid and eicosapentaenoic acid in polyunsaturated fatty acid metabolism in plaice neutrophils.
在鲽（一种海洋鱼类，其脂质富含(n-3)多不饱和脂肪酸）的外周血中性粒细胞中，研究了[1-14C]花生四烯酸和[1-14C]二十碳五烯酸掺入磷脂的情况。当单独以相等浓度向细胞提供这两种标记脂肪酸时，它们掺入磷脂的量大致相等。当两种酸都存在于培养液中时，花生四烯酸相对更受青睐。当按磷脂类别单位质量表示掺入量时，两种脂肪酸的掺入顺序均为磷脂酰肌醇（PI）＞磷脂酰胆碱（PC）＞磷脂酰乙醇胺（PE）＞磷脂酰丝氨酸（PS）＞鞘磷脂。然而，花生四烯酸掺入PI的特异性明显更高。二十碳五烯酸掺入PC的程度比花生四烯酸更高。结合花生四烯酸和二十碳五烯酸在鲽中性粒细胞多不饱和脂肪酸代谢中可能的作用对结果进行了讨论。
