Effect of catechins and high-temperature-processed green tea extract on scavenging reactive oxygen species and preventing Aβ fibrils' formation in brain microvascular endothelium.

The present study investigated the effect of high-temperature-processed green tea extract (HTP_GTE) and its bioactive components on the reduction of reactive oxygen species (ROS) and amyloid-beta (Aβ) protein in human microvascular endothelial cells. Compared to Aβ-only treatment, pretreatment of HTP_GTE was revealed to effectively inhibit ROS generation (<0.05). HTP_GTE and catechins not only inhibit Aβ fibril formation but also destabilize preformed Aβ fibrils. The presence of HTP_GTE, Aβ fibril formation was significantly inhibited in a dose-dependent manner at 12.5-100 μg/ml of HTP_GTE, showing 86-56%, respectively. Treatment of various concentrations of HTP_GTE and catechins steadily destabilized the preformed Aβ fibrils for 24 h in a dose-dependent manner. It was observed that the gallated groups such as epigallocatechin gallate, epicatechin gallate, gallocatechin gallate, and catechin gallate more effectively disturbed Aβ fibril formation and destabilized the preformed Aβ fibrils than the non-gallated group. Taken together, these findings supported that sterilized green tea could be promising natural anti-amyloidogenic agents associated with therapeutic approaches in Alzheimer's disease by scavenging ROS generation and Aβ fibril in the brain tissue.