Baxter R C, de Mellow J S
Clin Endocrinol (Oxf). 1986 Mar;24(3):267-78. doi: 10.1111/j.1365-2265.1986.tb03267.x.
A new radioreceptor assay for insulin-like growth factor-II (IGF-II), using receptors on ovine placental membranes, is described. Half-maximal displacement of specifically bound radioiodinated human IGF-II tracer was seen at 1.0 ng/tube of unlabelled IGF-II. The cross-reactivity of IGF-I was 1%, and insulin was entirely without effect. Measured on serum samples from 100 healthy adults, the mean IGF-II concentration (+/- SD) was 576 +/- 160 ng/ml. Identical mean values were seen for all adult age groups up to 65 years. The mean value for 10 acromegalic adults was 583 +/- 155 ng/ml, and for 9 GH-deficient subjects, 161 +/- 26 ng/ml (P less than 0.001 compared to normals). Of eight patients with chronic renal failure, none had an IGF-II level less than 2SD above the normal mean. No significant effect of renal dialysis was seen. In groups of patients with gastric, breast, lung, testicular, oat cell, ovarian, colonic and prostatic carcinoma, Hodgkin's lymphoma, non-Hodgkin's lymphoma, sarcoma and teratoma (5-12 patients per group), mean IGF-II levels were in the lower part of the normal range. Thus this study does not provide evidence supporting a role for excessive IGF-II production in the growth of any of these tumour types.
本文描述了一种利用绵羊胎盘膜上的受体检测胰岛素样生长因子-II（IGF-II）的新型放射受体分析方法。未标记的IGF-II浓度为1.0 ng/管时，可使特异性结合的放射性碘化人IGF-II示踪剂半数位移。IGF-I的交叉反应率为1%，胰岛素则完全无作用。对100名健康成年人的血清样本进行检测，IGF-II的平均浓度（±标准差）为576±160 ng/ml。65岁以下的所有成年年龄组的均值相同。10名肢端肥大症成年患者的均值为583±155 ng/ml，9名生长激素缺乏患者的均值为161±26 ng/ml（与正常人相比，P<0.001）。8名慢性肾衰竭患者中，无一例IGF-II水平低于正常均值2个标准差以上。未观察到肾透析的显著影响。在患有胃癌、乳腺癌、肺癌、睾丸癌、燕麦细胞癌、卵巢癌、结肠癌和前列腺癌、霍奇金淋巴瘤、非霍奇金淋巴瘤、肉瘤和畸胎瘤的患者组中（每组5-12例患者），IGF-II平均水平处于正常范围的下限。因此，本研究没有提供证据支持过量产生IGF-II在这些肿瘤类型的生长中起作用。
