Interdisciplinary Nanoscience Center (iNANO) , Aarhus University , Gustav Wieds Vej 14 , 8000 Aarhus , Denmark.
Department of Biomedicine , Aarhus University , Wilhelm Meyers Allé 3 , 8000 Aarhus , Denmark.
ACS Appl Mater Interfaces. 2018 Sep 12;10(36):30180-30190. doi: 10.1021/acsami.8b10886. Epub 2018 Aug 29.
Therapeutic cell mimicry aims to provide a source of cell-like assemblies, which exhibit the core structural or functional properties of their natural counterparts with broad envisioned applications in biomedicine. Bone tissue engineering (BTE) aims at promoting and inciting the natural healing process of, for instance, critically sized bone defects. Microreactors designed to co-assemble with biological bone-forming osteoblasts like SaOS-2 cells to start biomineralization are reported for the first time. The alginate-based microparticles are equipped with active alkaline phosphatase-loaded artificial liposomes or SaOS-2-derived matrix vesicles (MVs). Spheroids assembled from SaOS-2 cells and microreactors not only exhibit higher cell viability, but also show enhanced biomineralization when MVs are present. The active biomineralization stimulation of the microreactors is illustrated by colorimetric calcium quantification and micro-computed tomography. These findings show the promising potential of applying cell mimicry in BTE.
治疗性细胞模拟旨在提供类似细胞的组装体的来源，这些组装体表现出与其天然对应物的核心结构或功能特性，并在生物医学中有广泛的预期应用。骨组织工程（BTE）旨在促进和激发例如临界大小骨缺损的自然愈合过程。据报道，首次设计了与生物成骨成骨细胞（如 SaOS-2 细胞）共组装以开始生物矿化的微反应器。基于藻酸盐的微球配备了负载有碱性磷酸酶的人工脂质体或 SaOS-2 衍生的基质囊泡 (MVs)。由 SaOS-2 细胞和微反应器组装而成的球体不仅表现出更高的细胞活力，而且当存在 MVs 时还显示出增强的生物矿化作用。通过比色法钙定量和微计算机断层扫描说明了微反应器的主动生物矿化刺激作用。这些发现表明在 BTE 中应用细胞模拟的有前途的潜力。
