Haghjooy-Javanmard S, Ghasemi A, Laher I, Zarrin B, Dana N, Vaseghi G
Bratisl Lek Listy. 2018;119(4):229-233. doi: 10.4149/BLL_2018_043.
Morphine affects the risk of metastasis in cancer. The TLR4 gene promotes migration in adenocarcinoma cells. We investigated the effect of morphine on TLR4, MyD88 and NF-κ B-expression and migration. Migration of estrogen receptor-positive MCF7 breast cancer cells was studied after 24 and 48 hours incubation with morphine, with boyden chamber method. Morphine effect on TLR4, MyD88 and NF-κB mRNA expression was determined by quantitative Real-Time polymerase chain reaction. Migration was reduced at the doses of 0.5 and 5 µM (p < 0.05). However, TLR4, MyD88 and NF-κBmRNA expression was decreased at the doses of 0.5, 5 and 500 µM. Morphine at the dose of 50 µM increased the expression of mentioned genes. MCF-7 cell line after 48 hours incubation with the dose of 0.5 µM morphine decreased the migration and at the dose of 0.5 µM down-regulated the mRNA expression of TLR4, MyD88 and NF-κB, however, the higher doses increased the expression of TLR4, MyD88 and NF-κB. Morphine affects TLR4expression in breast cancer cell, which depends on time and concentration (Tab. 1, Fig. 5, Ref. 24).
吗啡影响癌症转移风险。TLR4基因促进腺癌细胞迁移。我们研究了吗啡对TLR4、MyD88和NF-κB表达及迁移的影响。采用博伊登小室法，在与吗啡孵育24小时和48小时后，研究雌激素受体阳性MCF7乳腺癌细胞的迁移情况。通过定量实时聚合酶链反应测定吗啡对TLR4、MyD88和NF-κB mRNA表达的影响。在0.5和5μM剂量下迁移减少（p<0.05）。然而，在0.5、5和500μM剂量下，TLR4、MyD88和NF-κB mRNA表达降低。50μM剂量的吗啡增加了上述基因的表达。用剂量为0.5μM的吗啡孵育48小时后的MCF-7细胞系迁移减少，且在0.5μM剂量下TLR4、MyD88和NF-κB的mRNA表达下调，然而，更高剂量则增加了TLR4、MyD88和NF-κB的表达。吗啡影响乳腺癌细胞中的TLR4表达，这取决于时间和浓度（表1，图5，参考文献24）。
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