Effects of Methadone on the Toll-like Receptor 4 Expression in Human Non-Small Cell Lung Carcinoma A549 Cell Line Using and Techniques.

BACKGROUND

In this study, the effects of methadone and naloxone on the expression of toll-like receptor 4 () gene have been evaluated in human non-small cell lung carcinoma A549 cell line migration using and techniques.

MATERIALS AND METHODS

Lung cancer A549 cell cultures were stimulated for 24 h with methadone (5, 10, and 20 μM) and naloxone (20 and 40 μM) concentrations. The level of expression was determined by the quantitative real-time polymerase chain reaction. Migration of the A549 cells was investigated after a 4-h incubation period with methadone using the Boyden Chamber assay.

RESULTS

Migration rate of the A549 cells treated with 5 ( < 0.05) and 20 ( < 0.01) μM methadone was, respectively, increased and decreased with 20 μM naloxone ( < 0.05). Furthermore, the expression was enhanced with 5 ( < 0.05) and 20 ( < 0.01) μM methadone and decreased with 20 ( < 0.05) and 40 μM naloxone ( < 0.01). In addition, docking analysis revealed docking of methadone to MD-2 and .

CONCLUSION

According to the present DATA, methadone affects the expression. It may however cause adverse consequences by increasing the expression. Therefore, the useful analgesic properties of methadone should be separated from the unwanted -mediated side effects.