Risk Assessment Division, Environmental Health Research Department, National Institute of Environmental Research, Incheon 22689, South Korea; Department of Chemical Engineering, Kwangwoon University, Seoul 01897, South Korea.
Risk Assessment Division, Environmental Health Research Department, National Institute of Environmental Research, Incheon 22689, South Korea.
Chemosphere. 2018 Oct;209:815-822. doi: 10.1016/j.chemosphere.2018.06.149. Epub 2018 Jun 26.
With the increase in the application of nano-consumer products containing engineered nanoparticles (NPs), the unintended environmental exposure to NPs has been inevitable. Because of the bioaccumulation of NPs, concern about their potential cytotoxicity to aquatic organisms is also growing. Although measuring tools for analyzing particle size and/or concentration of NPs in intracellular uptake of tissues have been well developed, a simultaneous analysis of the two characteristics is difficult. The objective of this study was to use single particle-inductively coupled plasma-mass spectrometry (sp-ICP-MS) to measure the bioaccumulation and particle size changes of NPs exposed to zebrafish (Danio rerio) for 7 days. The uptake of NPs in the liver, intestine, and gill tissues was confirmed by electron microscopic (EM) analysis. However, the primary particle size of NPs in tissues could not be determined by the EM analysis. Therefore, sp-ICP-MS coupled with alkaline digestion was used for the easy extraction and immediate analysis of NPs from tissues. Zebrafish were exposed to four NPs (30 and 80 nm gold/silver NPs; AuNPs/AgNPs). Uptake amounts of AgNPs in the liver and intestine were significantly higher than those of AuNPs. Although larger NPs were finally accumulated in the liver and intestine tissues, most of the smaller NPs were filtered in the gills. The sp-ICP-MS method coupled with alkaline digestion enabled the accurate analysis of size, size distribution, and mass concentration of NPs in an aquatic organism.
随着含有工程纳米粒子 (NPs) 的纳米消费品应用的增加， NPs 对环境的非故意暴露已不可避免。由于 NPs 的生物积累，人们对它们对水生生物潜在细胞毒性的担忧也在增加。虽然已经开发出用于分析组织内细胞摄取的 NPs 粒径和/或浓度的测量工具，但同时分析这两个特征是困难的。本研究的目的是使用单颗粒电感耦合等离子体质谱 (sp-ICP-MS) 测量暴露于斑马鱼 (Danio rerio) 7 天后 NPs 的生物积累和粒径变化。通过电子显微镜 (EM) 分析证实了 NPs 在肝脏、肠道和鳃组织中的摄取。然而，组织中 NPs 的原始粒径不能通过 EM 分析来确定。因此，使用 sp-ICP-MS 结合碱性消化法从组织中轻松提取和立即分析 NPs。将斑马鱼暴露于四种 NPs（30 和 80nm 金/银 NPs；AuNPs/AgNPs）。AgNPs 在肝脏和肠道中的摄取量明显高于 AuNPs。尽管较大的 NPs 最终在肝脏和肠道组织中积累，但大部分较小的 NPs 在鳃中被过滤。sp-ICP-MS 方法结合碱性消化可实现对水生生物中 NPs 的粒径、粒径分布和质量浓度的准确分析。
