camR，细胞色素P-450cam羟化酶操纵子的一个负调控基因座。
camR, a negative regulator locus of the cytochrome P-450cam hydroxylase operon.
作者信息
Koga H, Aramaki H, Yamaguchi E, Takeuchi K, Horiuchi T, Gunsalus I C
出版信息
J Bacteriol. 1986 Jun;166(3):1089-95. doi: 10.1128/jb.166.3.1089-1095.1986.
Abstract
A 4.27-kilobase insert from a HindIII DNA library of Pseudomonas putida carrying the CAM plasmid allowed coordinate expression of genes camD and camC under control of camR, an upstream regulator. The camC gene specifies cytochrome P-450cam, and camD specifies the 5-exo-alcohol dehydrogenase. A 1.38-kilobase deletion from the insert results in the constitutive expression of genes camC and camD; transformation in trans restores the substrate control, indicating that camR is a negative regulator.
摘要
携带CAM质粒的恶臭假单胞菌HindIII DNA文库中的一个4.27千碱基插入片段，使得camD和camC基因在camR（一种上游调节因子）的控制下能够协同表达。camC基因编码细胞色素P-450cam，camD基因编码5-外向醇脱氢酶。该插入片段缺失1.38千碱基会导致camC和camD基因组成型表达；反式转化可恢复底物控制，这表明camR是一种负调节因子。
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