camR，细胞色素P-450cam羟化酶操纵子的一个负调控基因座。
camR, a negative regulator locus of the cytochrome P-450cam hydroxylase operon.
作者信息
Koga H, Aramaki H, Yamaguchi E, Takeuchi K, Horiuchi T, Gunsalus I C
出版信息
J Bacteriol. 1986 Jun;166(3):1089-95. doi: 10.1128/jb.166.3.1089-1095.1986.
Abstract
A 4.27-kilobase insert from a HindIII DNA library of Pseudomonas putida carrying the CAM plasmid allowed coordinate expression of genes camD and camC under control of camR, an upstream regulator. The camC gene specifies cytochrome P-450cam, and camD specifies the 5-exo-alcohol dehydrogenase. A 1.38-kilobase deletion from the insert results in the constitutive expression of genes camC and camD; transformation in trans restores the substrate control, indicating that camR is a negative regulator.
摘要
携带CAM质粒的恶臭假单胞菌HindIII DNA文库中的一个4.27千碱基插入片段，使得camD和camC基因在camR（一种上游调节因子）的控制下能够协同表达。camC基因编码细胞色素P-450cam，camD基因编码5-外向醇脱氢酶。该插入片段缺失1.38千碱基会导致camC和camD基因组成型表达；反式转化可恢复底物控制，这表明camR是一种负调节因子。
相似文献
1
camR, a negative regulator locus of the cytochrome P-450cam hydroxylase operon.
J Bacteriol. 1986 Jun;166(3):1089-95. doi: 10.1128/jb.166.3.1089-1095.1986.
2
Cloning and nucleotide sequences of NADH-putidaredoxin reductase gene (camA) and putidaredoxin gene (camB) involved in cytochrome P-450cam hydroxylase of Pseudomonas putida.
J Biochem. 1989 Nov;106(5):831-6. doi: 10.1093/oxfordjournals.jbchem.a122939.
3
Evidence for autoregulation of camR, which encodes a repressor for the cytochrome P-450cam hydroxylase operon on the Pseudomonas putida CAM plasmid.
J Bacteriol. 1993 Dec;175(24):7828-33. doi: 10.1128/jb.175.24.7828-7833.1993.
4
Nucleotide sequence of the Pseudomonas putida cytochrome P-450cam gene and its expression in Escherichia coli.
J Biol Chem. 1986 Jan 25;261(3):1158-63.
5
Nucleotide sequence of the gene encoding a repressor for the cytochrome P-450cam hydroxylase operon on the Pseudomonas putida CAM plasmid.
Biochimie. 1994;76(1):63-70. doi: 10.1016/0300-9084(94)90064-7.
6
Purification and characterization of a cam repressor (CamR) for the cytochrome P-450cam hydroxylase operon on the Pseudomonas putida CAM plasmid.
J Bacteriol. 1995 Jun;177(11):3120-7. doi: 10.1128/jb.177.11.3120-3127.1995.
7
Complete nucleotide sequence of the 5-exo-hydroxycamphor dehydrogenase gene on the CAM plasmid of Pseudomonas putida (ATCC 17453).
Biochim Biophys Acta. 1993 Jul 18;1174(1):91-4. doi: 10.1016/0167-4781(93)90098-x.
8
Heterologous expression of the cytochrome P450cam hydroxylase operon and the repressor gene of Pseudomonas putida in Escherichia coli.
FEMS Microbiol Lett. 1994 Oct 15;123(1-2):49-54. doi: 10.1111/j.1574-6968.1994.tb07200.x.
9
Controlled and functional expression of the Pseudomonas oleovorans alkane utilizing system in Pseudomonas putida and Escherichia coli.
J Biol Chem. 1987 Dec 25;262(36):17712-8.
10
Transcription of the cam operon and camR genes in Pseudomonas putida PpG1.
J Bacteriol. 1993 Nov;175(21):6953-8. doi: 10.1128/jb.175.21.6953-6958.1993.
引用本文的文献
1
The TetR family of regulators.
Microbiol Mol Biol Rev. 2013 Sep;77(3):440-75. doi: 10.1128/MMBR.00018-13.
2
Camphor pathway redux: functional recombinant expression of 2,5- and 3,6-diketocamphane monooxygenases of Pseudomonas putida ATCC 17453 with their cognate flavin reductase catalyzing Baeyer-Villiger reactions.
Appl Environ Microbiol. 2013 May;79(10):3282-93. doi: 10.1128/AEM.03958-12. Epub 2013 Mar 22.
3
Cloning, Baeyer-Villiger biooxidations, and structures of the camphor pathway 2-oxo-Δ(3)-4,5,5-trimethylcyclopentenylacetyl-coenzyme A monooxygenase of Pseudomonas putida ATCC 17453.
Appl Environ Microbiol. 2012 Apr;78(7):2200-12. doi: 10.1128/AEM.07694-11. Epub 2012 Jan 20.
4
Cytochrome P450 is present in both ferrous and ferric forms in the resting state within intact Escherichia coli and hepatocytes.
J Biol Chem. 2011 Nov 25;286(47):40750-9. doi: 10.1074/jbc.M111.300871. Epub 2011 Oct 5.
5
Cloning, sequencing, and characterization of the hexahydro-1,3,5-Trinitro-1,3,5-triazine degradation gene cluster from Rhodococcus rhodochrous.
Appl Environ Microbiol. 2002 Oct;68(10):4764-71. doi: 10.1128/AEM.68.10.4764-4771.2002.
6
Evidence for autoregulation of camR, which encodes a repressor for the cytochrome P-450cam hydroxylase operon on the Pseudomonas putida CAM plasmid.
J Bacteriol. 1993 Dec;175(24):7828-33. doi: 10.1128/jb.175.24.7828-7833.1993.
7
Purification and characterization of a cam repressor (CamR) for the cytochrome P-450cam hydroxylase operon on the Pseudomonas putida CAM plasmid.
J Bacteriol. 1995 Jun;177(11):3120-7. doi: 10.1128/jb.177.11.3120-3127.1995.
8
Cosubstrate effects in reductive dehalogenation by Pseudomonas putida G786 expressing cytochrome P-450CAM.
Biodegradation. 1993;4(1):39-50. doi: 10.1007/BF00701453.
9
Transcription of the cam operon and camR genes in Pseudomonas putida PpG1.
J Bacteriol. 1993 Nov;175(21):6953-8. doi: 10.1128/jb.175.21.6953-6958.1993.
10
Purification and reconstitution of the electron transport components for 6-deoxyerythronolide B hydroxylase, a cytochrome P-450 enzyme of macrolide antibiotic (erythromycin) biosynthesis.
J Bacteriol. 1988 Apr;170(4):1548-53. doi: 10.1128/jb.170.4.1548-1553.1988.
本文引用的文献
1
Protein measurement with the Folin phenol reagent.
J Biol Chem. 1951 Nov;193(1):265-75.
2
LASTONE INTERMEDIATES IN THE MICROBIAL OXIDATION OF (+)-CAMPHOR.
Tetrahedron Lett. 1965 Mar 10;10:561-5. doi: 10.1016/s0040-4039(00)89996-7.
3
THE CARBON MONOXIDE-BINDING PIGMENT OF LIVER MICROSOMES. I. EVIDENCE FOR ITS HEMOPROTEIN NATURE.
J Biol Chem. 1964 Jul;239:2370-8.
4
An enzyme system for cyclic ketone lactonization.
Biochem Biophys Res Commun. 1961 Nov 29;6:293-7. doi: 10.1016/0006-291x(61)90382-5.
5
Enzymic synthesis of juvenile hormone in locust corpora allata: evidence for a microsomal cytochrome P-450 linked methyl farnesoate epoxidase.
Eur J Biochem. 1981 Aug;118(2):231-8. doi: 10.1111/j.1432-1033.1981.tb06391.x.
6
Amino acid sequence of the Pseudomonas putida cytochrome P-450. II. Cyanogen bromide peptides, acid cleavage peptides, and the complete sequence.
J Biol Chem. 1982 Nov 10;257(21):12664-71.
7
Rapid procedure for detection and isolation of large and small plasmids.
J Bacteriol. 1981 Mar;145(3):1365-73. doi: 10.1128/jb.145.3.1365-1373.1981.
8
Oxygen activation by cytochrome P-450.
Annu Rev Biochem. 1980;49:315-56. doi: 10.1146/annurev.bi.49.070180.001531.
9
Molecular and functional analysis of the TOL plasmid pWWO from Pseudomonas putida and cloning of genes for the entire regulated aromatic ring meta cleavage pathway.
Proc Natl Acad Sci U S A. 1981 Dec;78(12):7458-62. doi: 10.1073/pnas.78.12.7458.
10
Construction of a partial diploid for the degradative pathway encoded by the TOL plasmid (pWWO) from Pseudomonas putida mt-2: evidence for the positive nature of the regulation by the xyIR gene.
Mol Gen Genet. 1980 Jan;177(2):321-8. doi: 10.1007/BF00267445.
Zotero 插件