Yamaguchi T, Kaneda M, Kakinuma K
J Biochem. 1986 Mar;99(3):953-9. doi: 10.1093/oxfordjournals.jbchem.a135558.
Two laboratories (Borregaard et al. (1984) J. Biol. Chem. 259, 47; Ohno et al. (1985) J. Biol. Chem. 260, 2409) have reported that a b-type cytochrome (b558) was translocated into plasma membranes from specific granules in activated neutrophils. In an attempt to examine the cytochrome b translocation, porcine neutrophils were activated by treatment with surface-active agents such as myristate (MA) and phorbol myristate acetate (PMA), and then the postnuclear supernatants of both activated and unactivated cells were fractionated by Percoll density gradient centrifugation with a Zonal rotor. In activated neutrophils, high O2- generating activity was found in the plasma membrane fraction, which showed a peak of Na, K-ATPase activity as a marker enzyme. Cytochrome b558 was recovered 74 to 78% in the plasma membrane fraction and 14 to 16% in granules in either activated or unactivated cells. No change in specific content of cytochrome b558 was observed in plasma membranes before and after activation of cells. Furthermore, in both activated and unactivated cells, vitamin B12-binding protein, a specific granule marker, was mainly found in the bottom fractions and scarcely at all in plasma membranes. These results suggest that no translocation of cytochrome b558 occurs during activation of neutrophils.
两个实验室（Borregaard等人（1984年）《生物化学杂志》259卷，47页；Ohno等人（1985年）《生物化学杂志》260卷，2409页）报告称，一种b型细胞色素（b558）从活化中性粒细胞的特定颗粒转运到质膜中。为了检测细胞色素b的转运情况，用肉豆蔻酸盐（MA）和佛波醇肉豆蔻酸酯乙酸酯（PMA）等表面活性剂处理猪中性粒细胞使其活化，然后用区带转子通过Percoll密度梯度离心法对活化细胞和未活化细胞的核后上清液进行分级分离。在活化的中性粒细胞中，质膜部分具有高的O2生成活性，该部分显示出作为标记酶的钠钾ATP酶活性峰值。在活化或未活化的细胞中，细胞色素b558在质膜部分的回收率为74%至78%，在颗粒中的回收率为14%至16%。细胞活化前后，质膜中细胞色素b558的比含量未观察到变化。此外，在活化和未活化的细胞中，维生素B12结合蛋白，一种特定的颗粒标记物，主要存在于底部级分中，在质膜中几乎不存在。这些结果表明，中性粒细胞活化过程中细胞色素b558没有发生转运。
