长链非编码 RNA FTH1P3 通过 SP1/NF-κB 通路调控食管鳞癌细胞的转移和侵袭。
Long non-coding RNA FTH1P3 regulated metastasis and invasion of esophageal squamous cell carcinoma through SP1/NF-kB pathway.
机构信息
Department of Medical Laboratory, The First Affiliated Hospital, Zhengzhou University, Zhengzhou, Henan Province, China.
Institute of Medical and Pharmaceutical Science, Zhengzhou University, Zhengzhou, Henan Province, China.
出版信息
Biomed Pharmacother. 2018 Oct;106:1570-1577. doi: 10.1016/j.biopha.2018.07.129. Epub 2018 Jul 27.
UNLABELLED
AIMS：: Recent research showed that Long non-protein coding RNA ferritin heavy chain 1 pseudogene 3 (FTH1P3) plays a crucial role in the course of tumor formation. The present study was aimed to explore its role in esophageal squamous cell carcinoma (ESCC).
MAIN METHODS
Quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) was used to examine the expression levels of FTH1P3, mRNA SP1 and NF-kB in ESCC samples and cell lines. The impact of FTH1P3 knockdown was evaluated by WST-1 assays, colony formation assays, scratch wound assays, migration and invasion assays.
KEY FINDINGS
FTH1P3 was significantly upregulated in ESCC tissues and cells (P < 0.001). Knockdown of FTH1P3 notably decreased the proliferation, migration, and invasion capacity of ESCC cells. Silencing of FTH1P3 decreased the expression of specificity protein 1 (Sp1) and NF-kB (p65) in EC9706 and EC1.
SIGNIFICANCE
FTH1P3 plays a crucial role in ESCC tumorigenesis, and can be used as a potential therapeutic target for ESCC.
目的
最近的研究表明，长非蛋白编码 RNA 铁蛋白重链 1 假基因 3（FTH1P3）在肿瘤形成过程中起着至关重要的作用。本研究旨在探讨其在食管鳞状细胞癌（ESCC）中的作用。
方法
采用定量逆转录聚合酶链反应（qRT-PCR）检测 ESCC 样本和细胞系中 FTH1P3、mRNA SP1 和 NF-kB 的表达水平。通过 WST-1 检测、集落形成检测、划痕愈合检测、迁移和侵袭检测评估 FTH1P3 敲低的影响。
主要发现
FTH1P3 在 ESCC 组织和细胞中显著上调（P<0.001）。FTH1P3 的敲低显著降低了 ESCC 细胞的增殖、迁移和侵袭能力。沉默 FTH1P3 降低了 EC9706 和 EC1 中特异性蛋白 1（Sp1）和 NF-kB（p65）的表达。
意义
FTH1P3 在 ESCC 肿瘤发生中起着关键作用，可作为 ESCC 的潜在治疗靶点。
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