Department of Medical Laboratory, The First Affiliated Hospital, Zhengzhou University, Zhengzhou, Henan Province, China.
Institute of Medical and Pharmaceutical Science, Zhengzhou University, Zhengzhou, Henan Province, China.
Biomed Pharmacother. 2018 Oct;106:1570-1577. doi: 10.1016/j.biopha.2018.07.129. Epub 2018 Jul 27.
AIMS：: Recent research showed that Long non-protein coding RNA ferritin heavy chain 1 pseudogene 3 (FTH1P3) plays a crucial role in the course of tumor formation. The present study was aimed to explore its role in esophageal squamous cell carcinoma (ESCC).
Quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) was used to examine the expression levels of FTH1P3, mRNA SP1 and NF-kB in ESCC samples and cell lines. The impact of FTH1P3 knockdown was evaluated by WST-1 assays, colony formation assays, scratch wound assays, migration and invasion assays.
FTH1P3 was significantly upregulated in ESCC tissues and cells (P < 0.001). Knockdown of FTH1P3 notably decreased the proliferation, migration, and invasion capacity of ESCC cells. Silencing of FTH1P3 decreased the expression of specificity protein 1 (Sp1) and NF-kB (p65) in EC9706 and EC1.
FTH1P3 plays a crucial role in ESCC tumorigenesis, and can be used as a potential therapeutic target for ESCC.
最近的研究表明，长非蛋白编码 RNA 铁蛋白重链 1 假基因 3（FTH1P3）在肿瘤形成过程中起着至关重要的作用。本研究旨在探讨其在食管鳞状细胞癌（ESCC）中的作用。
采用定量逆转录聚合酶链反应（qRT-PCR）检测 ESCC 样本和细胞系中 FTH1P3、mRNA SP1 和 NF-kB 的表达水平。通过 WST-1 检测、集落形成检测、划痕愈合检测、迁移和侵袭检测评估 FTH1P3 敲低的影响。
FTH1P3 在 ESCC 组织和细胞中显著上调（P<0.001）。FTH1P3 的敲低显著降低了 ESCC 细胞的增殖、迁移和侵袭能力。沉默 FTH1P3 降低了 EC9706 和 EC1 中特异性蛋白 1（Sp1）和 NF-kB（p65）的表达。
FTH1P3 在 ESCC 肿瘤发生中起着关键作用，可作为 ESCC 的潜在治疗靶点。
