小鼠H-2基因复合体I-E区域内第二个重组热点的定位。
Mapping of a second recombination hot spot within the I-E region of the mouse H-2 gene complex.
作者信息
Lafuse W P, Berg N, Savarirayan S, David C S
出版信息
J Exp Med. 1986 Jun 1;163(6):1518-28. doi: 10.1084/jem.163.6.1518.
Abstract
The crossover points of nine intra-I region recombinant mouse strains were determined by restriction fragment analysis. The recombinants were examined for the presence of k and p haplotype specific DNA restriction endonuclease sites. These restriction sites were a Sac I site between the E beta and E beta 2 genes, a Hpa I site within the E beta 2 gene, and a Rsa I site approximately 1 kb to the right of the E alpha gene. Seven of the recombinants were found to have crossovers between the Hpa I and the Rsa I site. This analysis suggests that a recombination hot spot exists within this segment. This segment is approximately 12-14 kb, and contains the E alpha gene and the intervening sequence between the E beta 2 and E alpha genes.
摘要
通过限制性片段分析确定了9个I区内重组小鼠品系的交叉点。检测这些重组体中k和p单倍型特异性DNA限制性内切酶位点的存在情况。这些限制性位点分别是Eβ和Eβ2基因之间的Sac I位点、Eβ2基因内的Hpa I位点以及Eα基因右侧约1 kb处的Rsa I位点。发现其中7个重组体在Hpa I和Rsa I位点之间发生了交叉。该分析表明，在这一片段内存在一个重组热点。该片段约为12 - 14 kb，包含Eα基因以及Eβ2和Eα基因之间的间隔序列。
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