RecBC、sbcB独立，（AT）n介导的非洲爪蟾β珠蛋白基因侧翼序列在大肠杆菌中繁殖时的缺失。
RecBC, sbcB independent, (AT)n-mediated deletion of sequences flanking a Xenopus laevis beta globin gene on propagation in E. coli.
作者信息
Greaves D R, Patient R K
出版信息
Nucleic Acids Res. 1986 May 27;14(10):4147-58. doi: 10.1093/nar/14.10.4147.
Plasmids containing sequences 3' of the adult beta 1 globin gene of Xenopus laevis are unstable on propagation in a range of E. coli host strains. Up to 300 bp of Xenopus DNA are lost by rec A independent recombination between (AT)37 and (AT)17 sequences. Additionally, smaller deletions occurring in or around the (AT)37 sequence are observed. Deletion of these potential cruciform structures occurs in the absence of exonuclease I, exonuclease V and exonuclease VIII as the same pattern of deletion events is observed in recA recBC sbcB and recBC sbcA recE strains.
含有非洲爪蟾成年β1珠蛋白基因3'端序列的质粒在一系列大肠杆菌宿主菌株中传代时不稳定。通过（AT）37和（AT）17序列之间不依赖rec A的重组，多达300 bp的非洲爪蟾DNA会丢失。此外，还观察到在（AT）37序列中或其周围发生的较小缺失。这些潜在十字形结构的缺失在没有核酸外切酶I、核酸外切酶V和核酸外切酶VIII的情况下发生，因为在recA recBC sbcB和recBC sbcA recE菌株中观察到相同的缺失事件模式。
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