Graduate School of Applied Biological Sciences, Gifu University, 1-1 Yanagido, Gifu, Gifu, 501-1193, Japan.
Graduate School of Applied Biological Sciences, Gifu University, 1-1 Yanagido, Gifu, Gifu, 501-1193, Japan.
Chem Biol Interact. 2018 Oct 1;294:144-150. doi: 10.1016/j.cbi.2018.08.017. Epub 2018 Aug 18.
Currently, nanoparticles are used in various commercial products. One of the most common nanoparticles is titanium dioxide (TiO). It has a catalytic activity and UV absorption, and generates reactive oxygen species (ROS). This catalytic activity of TiO nanoparticles was believed to be capable of killing a wide range of microorganisms. In the environment, the unique properties of TiO nanoparticles can be maintained; therefore, the increasing use of TiO nanoparticles is raising concerns about their environmental risks. Thus, assessment of the biological and ecological effects of TiO-NOAAs is necessary. In this study, we assessed the effect of TiO-NOAAs for S. cerevisiae using DNA microarray. To compare yeast cells under various conditions, six treatment conditions were prepared (1. adsorbed fraction to TiO-NOAA under UV; 2. non-adsorbed fraction to TiO-NOAA under UV; 3. adsorbed fraction to TiO-NOAA without UV; 4. non-adsorbed fraction to TiO-NOAA without UV; 5. under UV; and 6. untreated control). The result of the DNA microarray analysis, suggested that yeast cells that are adsorbed by TiO-NOAA under UV irradiation suffer oxidative stress and this stress response was similar to that by only UV irradiation. We concluded that the effect of TiO-NOAAs on yeast cells under UV irradiation is not caused by TiO-NOAA but UV irradiation.
目前，纳米颗粒被应用于各种商业产品中。最常见的纳米颗粒之一是二氧化钛（TiO）。它具有催化活性和紫外线吸收能力，并产生活性氧物种（ROS）。这种 TiO 纳米颗粒的催化活性被认为能够杀死广泛的微生物。在环境中，TiO 纳米颗粒的独特性质可以得到保持；因此，TiO 纳米颗粒使用的增加引起了人们对其环境风险的关注。因此，评估 TiO-NOAAs 的生物和生态效应是必要的。在这项研究中，我们使用 DNA 微阵列评估了 TiO-NOAAs 对酿酒酵母的影响。为了比较不同条件下的酵母细胞，我们制备了六种处理条件（1. 在 UV 下吸附到 TiO-NOAA 的部分；2. 在 UV 下未吸附到 TiO-NOAA 的部分；3. 在没有 UV 的情况下吸附到 TiO-NOAA 的部分；4. 在没有 UV 的情况下未吸附到 TiO-NOAA 的部分；5. 在 UV 下；和 6. 未经处理的对照）。DNA 微阵列分析的结果表明，在 UV 照射下被 TiO-NOAA 吸附的酵母细胞遭受氧化应激，这种应激反应与仅受到 UV 照射的情况相似。我们得出结论，UV 照射下 TiO-NOAAs 对酵母细胞的影响不是由 TiO-NOAAs 引起的，而是由 UV 照射引起的。
