Badylak S F, Babbs C F
Resuscitation. 1986 Apr;13(3):165-73. doi: 10.1016/0300-9572(86)90098-5.
This study examined the effect of carbon dioxide, lidoflazine and deferoxamine therapy upon the 10-day survival incidence and subsequent neurologic function of rats subjected to 7 min of cardiorespiratory arrest with resuscitation. Cardiac arrest (asystole) was induced at time zero by injection of cold, 1% KCl into the left ventricle of ketamine-anesthetized rats pretreated with succinylcholine. Positive pressure ventilation was discontinued at time zero. Cardiopulmonary resuscitation (CPR) was begun at 7 min, and animals with return of spontaneous circulation were entered into the study. Twenty treated rats were ventilated for 1 h with 7% carbon dioxide-93% oxygen and given lidoflazine (2.0 mg/kg, i.v.) and deferoxamine (50 mg/kg, i.v.) 5 min after CPR. Twenty control rats were ventilated for 1 h with 100% oxygen and given lidoflazine vehicle and deferoxamine vehicle. Lidoflazine treatment (1.0 mg/kg) for the treated group, or lidoflazine vehicle for the control group, was repeated at 8 h postresuscitation. At 2 days postresuscitation, 75% of treated rats vs. 25% of control rats were alive (CHI2 = 10.0, d.f. = 1, P less than 0.01), and at 10 days, 60% of treated rats vs. 25% of control rats were alive (CHI2 = 5.01, d.f. = 1, P less than 0.05). There was no detectable neurologic deficit among survivors in either group at 15 days. The combination of carbon dioxide, lidoflazine and deferoxamine, administered after return of spontaneous circulation, is a simple and easily administered treatment regimen that improves the survival incidence without neurologic deficits in this animal model of cardiorespiratory arrest and CPR.
本研究检测了二氧化碳、利多氟嗪和去铁胺疗法对经历7分钟心肺骤停并复苏的大鼠10天存活发生率及随后神经功能的影响。在时间为零时，通过向预先用琥珀酰胆碱处理的氯胺酮麻醉大鼠的左心室注射冷的1%氯化钾诱导心脏骤停（心搏停止）。在时间为零时停止正压通气。在7分钟时开始心肺复苏（CPR），恢复自主循环的动物进入本研究。20只接受治疗的大鼠用7%二氧化碳 - 93%氧气通气1小时，并在心肺复苏后5分钟给予利多氟嗪（2.0毫克/千克，静脉注射）和去铁胺（50毫克/千克，静脉注射）。20只对照大鼠用100%氧气通气1小时，并给予利多氟嗪溶媒和去铁胺溶媒。复苏后8小时，对治疗组重复给予利多氟嗪治疗（1.0毫克/千克），对照组给予利多氟嗪溶媒。复苏后2天，75%的治疗组大鼠存活，而对照组为25%（卡方检验=10.0，自由度=1，P<0.01）；10天时，60%的治疗组大鼠存活，对照组为25%（卡方检验=5.01，自由度=1，P<0.05）。15天时，两组存活者均未检测到神经功能缺损。在这个心肺骤停和心肺复苏的动物模型中，自主循环恢复后给予二氧化碳、利多氟嗪和去铁胺的联合治疗是一种简单且易于实施的治疗方案，可提高存活发生率且无神经功能缺损。
