Plešinac S, Plećaš D, Babović I
Clinic for Obstetrics and Gynecology, Clinical Center of Serbia, Medical School University Belgrade, Majke Jevrosime 8, 11000 Belgrade, Serbia.
Indian J Hematol Blood Transfus. 2018 Jul;34(3):486-490. doi: 10.1007/s12288-018-0919-z. Epub 2018 Jan 24.
The aim of this research was to demonstrate: The specificity, sensitivity, the positive and negative prediction value of the PCR method in the detection of the fetal RhD status from maternal blood in various weeks of gestation. Which was the earliest gestational week, when is possible to get a reliable result? How did the presence of RhD antibodies in sensitized mothers affect the results? This was a prospective clinical study within a multidisciplinary project approved by the Ministry of Science of the Republic of Serbia, 2011. The study was approved by the Ethics Committee of the Medical Faculty in Belgrade. Ninety six RhD negative patients had participated in the study, with written consent. The sensitivity of the determination of the RhD status of the fetus from the mother's blood after the 7th GW in our sample was 98.6%, with a specificity of 92%. The positive predictive value of the test was 97% and the negative predictive value was 95.6%. False positive results were detected in 2.6% of cases and 4.5% of cases were false negative. This is a reliable test which should be used in monitoring of pregnancies of RhD negative mothers.
聚合酶链反应（PCR）方法在检测不同孕周孕妇血液中胎儿RhD状态时的特异性、敏感性、阳性预测值和阴性预测值。最早在哪个孕周可以获得可靠结果？致敏母亲体内RhD抗体的存在如何影响检测结果？这是一项前瞻性临床研究，属于塞尔维亚共和国科学部2011年批准的一个多学科项目。该研究经贝尔格莱德医学院伦理委员会批准。96名RhD阴性患者参与了本研究，并签署了书面同意书。在我们的样本中，孕7周后从母亲血液中检测胎儿RhD状态的敏感性为98.6%，特异性为92%。该检测的阳性预测值为97%，阴性预测值为95.6%。2.6%的病例检测到假阳性结果，4.5%的病例为假阴性。这是一项可靠的检测方法，应用于监测RhD阴性母亲的妊娠情况。
