使用下一代测序和生物信息学对慢性阻塞性肺疾病气道上皮的转录组图谱进行系统分析。
Systematic analysis of transcriptomic profiles of COPD airway epithelium using next-generation sequencing and bioinformatics.
作者信息
Chang Wei-An, Tsai Ming-Ju, Jian Shu-Fang, Sheu Chau-Chyun, Kuo Po-Lin
机构信息
Graduate Institute of Clinical Medicine, College of Medicine, Kaohsiung Medical University,
Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung Medical University,
出版信息
Int J Chron Obstruct Pulmon Dis. 2018 Aug 10;13:2387-2398. doi: 10.2147/COPD.S173206. eCollection 2018.
INTRODUCTION
COPD is a chronic inflammatory disease of lung. The inflammatory response in COPD is associated with neutrophils, macrophages, T lymphocytes, and bronchial epithelial cells, and occurs mainly in the small airway, leading to irreversible airflow limitation.
METHODS
In order to investigate the microRNA-mRNA interaction in the microenvironment of the COPD airway, we used next-generation sequencing and bioinformatics in this study.
RESULTS
We identified four genes with microRNA-mRNA interactions involved in COPD small-airway bronchial epithelial cells: , , , and . Furthermore, miR6511a-5p- interaction was found to be involved in small-airway bronchial epithelial cells, large-airway bronchial epithelial cells, and alveolar macrophages.
CONCLUSION
Our results showed that miR6511a-5p- interaction plays an important role in COPD, which might be associated with cell-cell contact, activation of leukocytes, activation of T lymphocytes, and cellular homeostasis. These findings provide new information for further investigations of the COPD microenvironment, and may help to develop new diagnostic or therapeutic strategies targeting the bronchial epithelium for COPD.
引言
慢性阻塞性肺疾病（COPD）是一种肺部慢性炎症性疾病。COPD中的炎症反应与中性粒细胞、巨噬细胞、T淋巴细胞和支气管上皮细胞有关，主要发生在小气道，导致不可逆的气流受限。
方法
为了研究COPD气道微环境中的微小RNA（miRNA）-信使核糖核酸（mRNA）相互作用，我们在本研究中使用了新一代测序和生物信息学方法。
结果
我们在COPD小气道支气管上皮细胞中鉴定出四个存在miRNA-mRNA相互作用的基因： 、 、 和 。此外，发现miR6511a-5p-相互作用涉及小气道支气管上皮细胞、大气道支气管上皮细胞和肺泡巨噬细胞。
结论
我们的结果表明，miR6511a-5p-相互作用在COPD中起重要作用，这可能与细胞间接触、白细胞激活、T淋巴细胞激活和细胞稳态有关。这些发现为进一步研究COPD微环境提供了新信息，并可能有助于开发针对COPD支气管上皮的新诊断或治疗策略。
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