Mackay J A, Sass-Kuhn S, Moqbel R, Walsh G M, Kay A B
Allergy. 1986 Apr;41(3):169-78. doi: 10.1111/j.1398-9995.1986.tb00297.x.
Human granulocyte/pollen binding protein (GPBP), previously identified as serum transferrin, promoted prolonged firm adherence of neutrophils to Timothy grass pollen. Some characteristics of this adherence reaction are reported. GPBP-induced binding was time-, temperature- and concentration-dependent. Maximal adherence was observed by 2 h and was only slightly decreased at 18 h. The optimal temperature for adherence was 37 degrees C. Concentrations of GPBP as low as 1.25 microgram/ml gave significantly greater binding than the albumin or lactoferrin control. Eosinophils, monocytes and lymphocytes did not appear to participate in GPBP-induced pollen binding reactions at concentrations up to 300 micrograms/ml. In the presence of GPBP, neutrophils adhered to a range of grass, weed and tree pollens. These included timothy, meadow, false oat, rye, giant and short ragweed, plantain, silver birch and ash. GPBP did not facilitate the adherence of granulocytes to inert particles of similar size such as Sephadex beads and agarose. The adherence was Mg++- but not Ca++-dependent and was not inhibited by a monoclonal antibody to the transferrin receptor (OKT9). Transferrin/GPBP did not bind to either neutrophils or pollen grains. A purified commercial transferrin reacted in all respects like GPBP in these pollen binding studies. These observations indicate that GPBP/transferrin-induced adherence of granulocytes to pollen grains is a hitherto unrecognized property of transferrin which appears unrelated to iron transport or the conventional transferrin receptor.
人类粒细胞/花粉结合蛋白（GPBP），先前被鉴定为血清转铁蛋白，可促进中性粒细胞与梯牧草花粉的长时间牢固黏附。本文报道了这种黏附反应的一些特征。GPBP诱导的结合具有时间、温度和浓度依赖性。在2小时时观察到最大黏附，在18小时时仅略有下降。黏附的最佳温度为37摄氏度。低至1.25微克/毫升的GPBP浓度所产生的结合显著高于白蛋白或乳铁蛋白对照。在浓度高达300微克/毫升时，嗜酸性粒细胞、单核细胞和淋巴细胞似乎未参与GPBP诱导的花粉结合反应。在GPBP存在的情况下，中性粒细胞可黏附于一系列禾本科、杂草和树木花粉。这些花粉包括梯牧草、草地早熟禾、假燕麦、黑麦、巨型豚草和短叶豚草、车前草、白桦和白蜡树。GPBP并未促进粒细胞与类似大小的惰性颗粒（如葡聚糖凝胶珠和琼脂糖）的黏附。这种黏附依赖于Mg++而非Ca++，且不受转铁蛋白受体单克隆抗体（OKT9）的抑制。转铁蛋白/GPBP既不与中性粒细胞结合，也不与花粉粒结合。在这些花粉结合研究中，一种纯化的商业转铁蛋白在所有方面的反应都与GPBP相似。这些观察结果表明，GPBP/转铁蛋白诱导的粒细胞与花粉粒的黏附是转铁蛋白迄今未被认识的特性，这一特性似乎与铁运输或传统的转铁蛋白受体无关。
