Tyler M S
Arch Oral Biol. 1986;31(4):255-9. doi: 10.1016/0003-9969(86)90057-9.
Mandibles were cultured in the presence of dibutyryl cyclic AMP (1 mM) and theophylline (1 mM) and in control medium. Controls differentiated normally but the timing of events differed from that in ovo and feathers did not form. With elevated intracellular cyclic-AMP levels, membrane bone did not form in the earlier stages tested and was inhibited or reduced in older ones; chondrogenesis was inhibited only in young explants (HH stage 20) and, in certain instances, it was enhanced. There was precocious and hyperplastic differentiation of mucous cells within oral epithelium but the aboral epithelium was unchanged.
将下颌骨置于含有二丁酰环磷酸腺苷（1 mM）和茶碱（1 mM）的培养基中以及对照培养基中进行培养。对照组正常分化，但事件发生的时间与在鸡胚内不同，且未形成羽毛。随着细胞内环磷酸腺苷水平升高，在早期测试阶段膜性骨未形成，而在较老阶段受到抑制或减少；软骨形成仅在年轻外植体（HH 阶段 20）中受到抑制，且在某些情况下有所增强。口腔上皮内的黏液细胞出现早熟和增生性分化，但背侧上皮未发生变化。
