McQuade R, Thomas C G, Nayfeh S N
Biochem Biophys Res Commun. 1986 May 29;137(1):61-8. doi: 10.1016/0006-291x(86)91175-7.
Following cross-linking with disuccinimidyl suberate and analysis by SDS-PAGE and autoradiography, both the high- and low-affinity TSH binding components exhibited two similar 125I-TSH-labeled bands, with Mr values of 80,000 and 68,000. IgG fractions from patients with Graves' disease inhibited 125I-TSH binding to both components, while normal IgG had no effect. Although not entirely conclusive, these results suggest that the high- and low-affinity components share similar subunit composition and antigenic determinants.
在用辛二酸二琥珀酰亚胺酯进行交联并通过SDS-PAGE和放射自显影分析后，高亲和力和低亲和力促甲状腺激素结合成分均显示出两条相似的125I-促甲状腺激素标记带，其Mr值分别为80,000和68,000。格雷夫斯病患者的IgG组分可抑制125I-促甲状腺激素与这两种成分的结合，而正常IgG则无此作用。尽管这些结果并非完全确凿，但表明高亲和力和低亲和力成分具有相似的亚基组成和抗原决定簇。
