Boles T C, Hogan M E
Biochemistry. 1986 May 20;25(10):3039-43. doi: 10.1021/bi00358a045.
We have used a photochemical method to map covalent binding sites of the carcinogen benzo[a]pyrenediol epoxide (BPDE) within DNA from the transcriptional control region of the chicken adult beta-globin gene. Our preliminary low-resolution mapping has demonstrated that this region contains highly preferred BPDE binding sites [Boles, T.C., & Hogan, M.E. (1984) Proc. Natl. Acad. Sci. U.S.A. 81, 5623-5627]. Here, we find that BPDE binding at individual G residues in this region is influenced by nearest-neighbor interactions and also by longer range interactions that may be attributable to sequence-specific variation of DNA secondary structure. Our findings suggest that long poly(dG) sequences should be preferred sites for BPDE action in other genes.
我们采用了一种光化学方法来绘制致癌物苯并[a]芘二醇环氧化物（BPDE）在鸡成年β-珠蛋白基因转录控制区域的DNA内的共价结合位点。我们初步的低分辨率图谱表明，该区域包含高度优先的BPDE结合位点[博尔斯，T.C.，& 霍根，M.E.（1984年）美国国家科学院院刊81，5623 - 5627]。在此，我们发现该区域单个G残基处的BPDE结合受到最近邻相互作用以及可能归因于DNA二级结构序列特异性变化的更远距离相互作用的影响。我们的研究结果表明，长的聚（dG）序列应该是其他基因中BPDE作用的优先位点。
