Zhang Lei, Zhou Jie, Luo Jun, Wang Qiong, Liu Jia, Zeng Qing-qi
Zhong Yao Cai. 2016 May;39(5):1134-8.
To observe the effect and mechanism of mulberry anthocyanins on autophagy and apoptosis in SGC-7901 cells.
MTT assay was used to detected mulberry anthocyanins on proliferation of SGC-7901 cells; flow cytometry analysis was used to detected the influence of mulberry anthocyanins on cell apoptosis; Hoechst 33342 / PI live cell staining was used to observe the morphological changes of apoptotic nuclei; transmission electron microscope( TEM) was used to observe the ultrastructural changes of the SGC-7901 cells. Expression of LC3,Beclin1,BAX,BCL-2 and Caspase-8 in SGC-7901 cells were detected by Western blot.
Mulberry anthocyanins can inhibit the proliferation of SGC-7901 cells, and mulberry anthocyanins can induce cells apoptosis by the results of flow cytometry and Hoechst33342 / PI double staining. TEM observation results indicated that mulberry anthocyanins intervented SGC-7901 cells induced autophagy. The result of Western blot confirmed that mulberry anthocyanins can increase the ratio of LC3-Ⅱ /LC3-Ⅰ,BAX / BCL-2 ratio and the expression of Caspase-8,Beclin1 of SGC-7901 cells.
Mulberry anthocyanins can inhibit the proliferation of human gastric cancer SGC-7901 cells, inducing cell apoptosis and autophagy. The mechanism probably relate to the up-regulating the ratio of BAX / BCL-2 and increasing the expression of Beclin1 and Caspase-8.
观察桑椹花青素对SGC-7901细胞自噬和凋亡的影响及其机制。
采用MTT法检测桑椹花青素对SGC-7901细胞增殖的影响；采用流式细胞术分析检测桑椹花青素对细胞凋亡的影响；采用Hoechst 33342/PI活细胞染色观察凋亡细胞核的形态变化；采用透射电子显微镜（TEM）观察SGC-7901细胞的超微结构变化。通过蛋白质免疫印迹法检测SGC-7901细胞中LC3、Beclin1、BAX、BCL-2和Caspase-8的表达。
桑椹花青素可抑制SGC-7901细胞的增殖，流式细胞术和Hoechst33342/PI双重染色结果表明桑椹花青素可诱导细胞凋亡。TEM观察结果表明桑椹花青素干预SGC-7901细胞可诱导自噬。蛋白质免疫印迹法结果证实桑椹花青素可增加SGC-7901细胞中LC3-Ⅱ/LC3-Ⅰ的比值、BAX/BCL-2比值以及Caspase-8、Beclin1的表达。
桑椹花青素可抑制人胃癌SGC-7901细胞的增殖，诱导细胞凋亡和自噬。其机制可能与上调BAX/BCL-2比值以及增加Beclin1和Caspase-8的表达有关。
