Fang Yuan, Shi Wen-qi, Zhang Yi, Hu Qiu-an, Zhou Zheng-bin, Wu Jia-tong, Zhang Liang-liang
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 2017 Feb;35(1):31-5.
To record the discovery of Culex inatomii in Chongming, Shanghai.
Larvae and adult mosquitos of Cx. inatomii were collected in Dongtan of Chongming Island from May to November in 2015 and 2016, and their morphological characteristics were observed. The genomic DNA was extracted from adult mosquitos, PCR was performed to amplify the cytochrome c oxidaseⅠ(COⅠ) gene. Multiple alignment of COⅠ sequence was conducted with ClustalW2. Pairwise distances within and between species were calculated using MEGA v5.10 based on COⅠ sequences of Cx. inatomii, Cx. modestus, Cx. pipiens pallens, and Cx. tritaeniorhynchus. The phylogenetic tree of the above four species was constructed using neighbor joining, maximum likelihood, and Bayesian methods.
One hundred and fifty-six adult female mosquitos and 36 larvae of Cx. inatomii were collected. Larvae were reared to adult stage in the laboratory (17 female, 19 male). Morphologically, the subapical lobe of the sidepiece in male genitalia was divided into two parts, the anterior part having 2 bladed setae, and the posterior part having 1 bladed setae and 1 lanceolar strong setae. This strucutre can be used to distinguish Cx. inatomii from Cx. modestus. PCR of COⅠ resulted in products of approximately 650 bp. They were sequenced and the sequencing result was submitted to GenBank (accession number, KX555565-KX555570). Multiple sequence alignment revealed a 96% sequence similarity of COⅠ between Cx. inatomii and Cx. modestus. The genetic distance between Cx. inatomii and Cx. modestus was 0.047, and that within them each was 0.003 and 0.011, respectively. The phylogenetic tree showed that the four species clustered as a monophyletic clade, and each formed an individual lineage. Cx. inatomii had a closer relationship with Cx. modestus, while distant from the other two species.
We recorded the discovery of Cx. inatomii in Chongming, Shanghai.
记录在上海崇明发现的伊蚊库蚊。
于2015年和2016年5月至11月在崇明岛东滩采集伊蚊库蚊的幼虫和成虫，观察其形态特征。从成虫中提取基因组DNA，进行PCR扩增细胞色素c氧化酶Ⅰ（COⅠ）基因。使用ClustalW2对COⅠ序列进行多重比对。基于伊蚊库蚊、中型库蚊、淡色库蚊和三带喙库蚊的COⅠ序列，使用MEGA v5.10计算种内和种间的成对距离。使用邻接法、最大似然法和贝叶斯方法构建上述四种库蚊的系统发育树。
采集到156只伊蚊库蚊成年雌蚊和36只幼虫。幼虫在实验室饲养至成虫阶段（17只雌蚊，19只雄蚊）。形态学上，雄性生殖器侧叶的亚顶端叶分为两部分，前部有2根叶片状刚毛，后部有1根叶片状刚毛和1根矛状强刚毛。这种结构可用于区分伊蚊库蚊和中型库蚊。COⅠ的PCR扩增产生约650 bp的产物。对其进行测序，并将测序结果提交至GenBank（登录号，KX555565-KX555570）。多重序列比对显示伊蚊库蚊和中型库蚊的COⅠ序列相似性为96%。伊蚊库蚊和中型库蚊之间的遗传距离为0.047，它们各自内部的遗传距离分别为0.003和0.011。系统发育树显示这四种库蚊聚为一个单系类群，且各自形成一个独立的谱系。伊蚊库蚊与中型库蚊的关系更近，而与其他两种库蚊关系较远。
我们记录了在上海崇明发现伊蚊库蚊。
