School of Public Health , Jilin University , Changchun 130021 , China.
The Department of Cadre Ward , The First Hospital of Jilin University , Changchun 130021 , China.
J Agric Food Chem. 2018 Sep 12;66(36):9516-9521. doi: 10.1021/acs.jafc.8b02494. Epub 2018 Aug 29.
Vibrio parahemolyticus ( V. parahemolyticus) is an important food-borne pathogen that causes food poisoning and acute gastroenteritis in humans. Herein, a novel colorimetric immunoassay was presented for rapid detection of V. parahemolyticus using gold nanoparticles (18.1 nm diameter) as chromogenic substrate, whose combination of a magnetic bead-based sandwich immunoassay and an optical sensing system via Mn ions mediated aggregation of gold nanoparticles. MnO nanoparticles coated with polyclonal IgG antibodies (7.8 nm diameter) are used to recognize the target and can be etched to generate manganese ions by ascorbic acid. A color change ranging from red to purple to blue can be easily discerned by bare eye, corresponding to V. parahemolyticus concentration in the range between 10 and 10 cfu·mL. The proposed method possesses high specificity with a limit of detection of 10 cfu·mL and was successfully applied to determination of V. parahemolyticus in oyster samples without pre-enrichment. In our perception, it shows promise in rapid instrumental and on-site visual detection of V. parahemolyticus.
副溶血性弧菌（Vibrio parahemolyticus）是一种重要的食源性致病菌，可引起人类食物中毒和急性肠胃炎。本研究提出了一种快速检测副溶血性弧菌的比色免疫分析方法，该方法以金纳米粒子（直径 18.1nm）作为显色底物，结合基于磁珠的夹心免疫分析和通过 Mn 离子介导的金纳米粒子聚集的光学传感系统。用 MnO 纳米粒子（直径 7.8nm）包被多克隆 IgG 抗体，用于识别目标物，并可通过抗坏血酸进行刻蚀以生成锰离子。通过裸眼可轻松识别从红色到紫色到蓝色的颜色变化，对应于 10 至 10 cfu·mL 范围内的副溶血性弧菌浓度。该方法具有较高的特异性，检测限为 10 cfu·mL，并且无需预富集即可成功应用于牡蛎样品中副溶血性弧菌的测定。在我们看来，它有望实现副溶血性弧菌的快速仪器和现场可视化检测。
