Kamesaki H, Fukuhara S, Tatsumi E, Uchino H, Yamabe H, Miwa H, Shirakawa S, Hatanaka M, Honjo T
Blood. 1986 Jul;68(1):285-92.
A novel cell line, KM-H2, was established from the pleural effusion of a patient with Hodgkin's disease of mixed cellular type. Multiple phenotypic studies were carried out with this cell line. Acid phosphatase and nonspecific esterase activities were detected. Rosette formation with T lymphocytes and the receptors for C3b and Fc portion of IgG were positive. Among the antigens tested with a total of 22 monoclonal antibodies defining hematopoietic cell subsets or lineages, Ki-1, Leu-M1, MCS1, HLA-DR, and OKT9 antigens were found to be positive. The other antigens reportedly specific for T cells, B cells, natural killer (NK) cells, monocytes, interdigitating reticulum (IR) cells and dendritic reticulum cells were negative. These phenotypic features were identical to those of the Sternberg-Reed (SR) and Hodgkin (H) cells in the fresh materials reported by other researchers. Moreover, the KM-H2 cells and the parental pleural effusion cells shared several structural chromosome anomalies. These findings indicated that the KM-H2 cells are derived from the SR and H cells. Molecular genetic analysis of the KM-H2 cells disclosed that the human immunoglobulin JH gene was rearranged but not the JK gene, and that the human T cell receptor beta chain gene was of the germline type. Based on these properties of the KM-H2 cells, Hodgkin's disease may be derived from a cell lineage other than T cell or B cell.
一种新的细胞系KM-H2是从一名混合细胞型霍奇金病患者的胸腔积液中建立的。对该细胞系进行了多项表型研究。检测到酸性磷酸酶和非特异性酯酶活性。与T淋巴细胞形成玫瑰花结以及对IgG的C3b和Fc部分的受体呈阳性。在用总共22种定义造血细胞亚群或谱系的单克隆抗体检测的抗原中，发现Ki-1、Leu-M1、MCS1、HLA-DR和OKT9抗原呈阳性。据报道，其他对T细胞、B细胞、自然杀伤（NK）细胞、单核细胞、交错突网状（IR）细胞和树突状网状细胞特异的抗原为阴性。这些表型特征与其他研究人员报道的新鲜材料中的施特恩伯格-里德（SR）细胞和霍奇金（H）细胞的表型特征相同。此外，KM-H2细胞和亲本胸腔积液细胞共有几个结构染色体异常。这些发现表明，KM-H2细胞来源于SR细胞和H细胞。对KM-H2细胞的分子遗传学分析表明，人免疫球蛋白JH基因发生了重排，但JK基因未重排，并且人T细胞受体β链基因属于种系类型。基于KM-H2细胞的这些特性，霍奇金病可能来源于T细胞或B细胞以外的细胞谱系。
