Silva Bruna Fernanda da, Bassetto César Cristiano, Amarante Alessandro Francisco Talamini do
Programa de Pós-graduação em Ambiente e Saúde, Universidade do Planalto Catarinense - UNIPLAC, Lages, SC, Brasil.
Departamento de Parasitologia, Instituto de Biociências, Universidade Estadual Paulista - UNESP, Botucatu, SP, Brasil.
Rev Bras Parasitol Vet. 2018 Jul-Sep;27(3):295-300. doi: 10.1590/S1984-296120180045. Epub 2018 Aug 16.
Twenty-six newborn lambs were evaluated for 21 weeks, from birth to slaughter, to assess their plasma anti-Oestrus ovis immunoglobulin (IgG) using the ELISA technique. On the last day of sampling, all the lambs were slaughtered and O. ovis larvae were recovered, quantified and identified according to the larval stage. High levels of IgG were observed over the first three weeks of life, thus indicating that antibodies are transferred via colostrum from ewes to lambs. Afterwards, the antibody levels declined progressively until the lambs were 11 weeks of age and subsequently started to increase again when they were around 13 weeks of age, reaching the apex on the last week of sampling. All the lambs were parasitized with different larval stages of O. ovis, with an average of 39 larvae per lamb, and the intensity of the infestation ranged from 10 to 97 larvae. However, there was non-significant correlation coefficients between IgG levels and O. ovis larval burden (P > 0.05). In conclusion, although the lambs became infested with O. ovis at an early age, the larval burden was not associated with specific IgG levels.
对26只新生羔羊从出生到屠宰进行了21周的评估，采用酶联免疫吸附测定（ELISA）技术检测其血浆抗羊狂蝇免疫球蛋白（IgG）。在采样的最后一天，所有羔羊均被屠宰，并回收羊狂蝇幼虫，根据幼虫阶段进行定量和鉴定。在出生后的前三周观察到高水平的IgG，这表明抗体通过初乳从母羊转移到羔羊体内。此后，抗体水平逐渐下降，直到羔羊11周龄，随后在13周龄左右又开始上升，在采样的最后一周达到峰值。所有羔羊均感染了不同幼虫阶段的羊狂蝇，每只羔羊平均有39条幼虫，感染强度为10至97条幼虫。然而，IgG水平与羊狂蝇幼虫负荷之间的相关系数无统计学意义（P>0.05）。总之，尽管羔羊在幼年时就感染了羊狂蝇，但幼虫负荷与特定的IgG水平无关。
