Blecha Isabella Maiumi Zaidan, Csordas Bárbara Guimarães, Aguirre André de Abreu Rangel, Cunha Rodrigo Casquero, Garcia Marcos Valério, Andreotti Renato
Laboratório de Biologia do Carrapato, Embrapa Gado de Corte, Empresa Brasileira de Pesquisa Agropecuária - EMBRAPA, Campo Grande, MS, Brasil.
Programa de Pós-graduacão em Ciência Animal, Faculdade de Medicina Veterinária e Zootecnia - FAMEZ, Universidade Federal de Mato Grosso do Sul - UFMS, Campo Grande, MS, Brasil.
Rev Bras Parasitol Vet. 2018 Jul-Sep;27(3):267-279. doi: 10.1590/S1984-296120180056. Epub 2018 Aug 16.
The cattle tick Rhipicephalus microplus causes significant economic losses in agribusiness. Control of this tick is achieved mainly through the application of chemical acaricides, often resulting in contamination of animal food products and of the environment. Another major concern associated with acaricide use is the increasing reports of resistance of this tick vector against the active ingredients of many commercial products. An alternative control method is vaccination. However, the commercially available vaccine based on a protein homologous to Bm86 exhibits variations in efficacy relative to the different geographical locations. This study aimed to identify antigenic determinants of the sequences of proteins homologous to Bm86. Phylogenetic analyses were performed to determine the extent of divergence between different populations of R. microplus to identify the sequence that could be used as a universal vaccine against the multiple geographically distinct populations of R. microplus and related tick species. Considering the extensive sequence and functional polymorphism observed among strains of R. microplus from different geographical regions, we can conclude that it may be possible to achieve effective vaccination against these cattle ticks using a single universal Bm86-based antigen.
微小牛蜱（Rhipicephalus microplus）给农业综合企业造成了重大经济损失。对这种蜱虫的控制主要通过施用化学杀螨剂来实现，这常常导致动物食品和环境受到污染。与使用杀螨剂相关的另一个主要问题是，关于这种蜱虫媒介对许多商业产品活性成分产生抗药性的报道日益增多。一种替代控制方法是接种疫苗。然而，基于与Bm86同源的蛋白质的市售疫苗在不同地理位置的效力存在差异。本研究旨在确定与Bm86同源的蛋白质序列的抗原决定簇。进行了系统发育分析，以确定不同微小牛蜱种群之间的差异程度，从而确定可作为针对多个地理上不同的微小牛蜱种群及相关蜱种的通用疫苗的序列。考虑到在来自不同地理区域的微小牛蜱菌株中观察到的广泛序列和功能多态性，我们可以得出结论，使用单一基于Bm86的通用抗原来实现针对这些牛蜱的有效疫苗接种或许是可行的。
