Peconick A P, Sossai S, Girão F A, Rodrigues M Q R B, Souza E Silva C H, Guzman Q F, Patarroyo V A M, Vargas M I, Patarroyo J H
Laboratório de Biologia e Controle de Hematozoários e Vetores, DVT, Instituto de Biotecnologia Aplicada A Agropecuária-BIOAGRO, Universidade Federal de Viçosa-BIOAGRO, Campus Universitario, 36570-000 Viçosa, MG, Brazil.
Exp Parasitol. 2008 May;119(1):37-43. doi: 10.1016/j.exppara.2007.12.007. Epub 2007 Dec 23.
The synthetic vaccine SBm7462 is based on three immunogenic epitopes (4822, 4823 and 4824) contained within protein Bm86 derived from the Australian Yeerongpilly strain of the tick Rhipicephalus (Boophilus) microplus. Twenty strains of the tick originating from Brazil, Argentina, Colombia and Uruguay were analysed in order to identify differences compared with sequences present in components of vaccine SBm7462. For each parasite population, three cDNA fragments containing the nucleotides coding for the epitopes 4822, 4824 and 4823 were sequenced, and the amino acid sequences were deduced and compared with those of the homologous bm86 gene. The results indicate that the epitope sequences of vaccine SBm7462 are conserved in the South American populations of the tick. The conservation of such sequences is very important for the immunological response of different populations of R. (B.) microplus.
合成疫苗SBm7462基于来源于澳大利亚微小牛蜱（Rhipicephalus (Boophilus) microplus）耶龙皮利菌株的蛋白Bm86中包含的三个免疫原性表位（4822、4823和4824）。对源自巴西、阿根廷、哥伦比亚和乌拉圭的20个蜱虫菌株进行了分析，以确定与疫苗SBm7462各组分中存在的序列相比的差异。对于每个寄生虫群体，对包含编码表位4822、4824和4823的核苷酸的三个cDNA片段进行了测序，并推导了氨基酸序列，并与同源bm86基因的氨基酸序列进行了比较。结果表明，疫苗SBm7462的表位序列在南美洲蜱虫群体中是保守的。这些序列的保守性对于微小牛蜱不同群体的免疫反应非常重要。
