Public Employee of Sao Paulo Hospital (IAMSPE), Sao Paulo, Brazil.
Department of Medicine (LIM 20), School of Medicine, University of Sao Paulo, Sao Paulo, Brazil.
Sci Rep. 2018 Aug 22;8(1):12636. doi: 10.1038/s41598-018-30851-6.
Work-exacerbated asthma (WEA) is defined as preexisting asthma that worsens with exposure to irritants [e.g., chlorine (Cl) derivatives] in the workplace. The maximum allowable concentration in the workplace of Cl exposure is 3 mg/ m (described in OSHA). We investigated in an experimental asthma model in mice the effects of a single exposure to a sodium hypochlorite dose with this allowed chlorine concentration and a tenfold higher dose. Acute chlorine exposure at 3.3 mg/m in the OVA-sensitized group increased eosinophils in the peribronquial infiltrate, cytokine production, nasal mucus production and the number of iNOS positive cells in the distal lung compared to only sensitized mice. The exposure to a higher dose of 33.3 mg/m in the OVA-sensitized group resulted in an increase in respiratory system elastance, in the total and differential numbers of inflammatory cells in bronchoalveolar lavage fluid, IL-4, IL-5, and IL-17 in the lungs, eosinophils in peribronquial infiltrate and mucus content in nasal compared to non-exposed and sensitized animals. In this asthma model, chorine exposures at an allowable dose, contributed to the potentiation of Th2 responses. The functional alterations were associated with increased iNOS and ROCK-2 activation in the distal lung.
工作相关哮喘（WEA）定义为既往存在的哮喘，在工作场所暴露于刺激物（如氯衍生物）时会恶化。工作场所的氯暴露最大允许浓度为 3mg/m（OSHA 规定）。我们在实验性哮喘小鼠模型中研究了单次接触允许浓度的次氯酸钠剂量和十倍更高剂量的氯的影响。与仅致敏的小鼠相比，在 OVA 致敏组中，3.3mg/m 的急性氯暴露增加了周边支气管浸润中的嗜酸性粒细胞、细胞因子产生、鼻黏液产生和远端肺中 iNOS 阳性细胞的数量。在 OVA 致敏组中，暴露于 33.3mg/m 的更高剂量会导致呼吸系统弹性增加、支气管肺泡灌洗液中总细胞和差异细胞数量增加、肺中 IL-4、IL-5 和 IL-17 增加、周边支气管浸润中的嗜酸性粒细胞增加以及与非暴露和致敏动物相比，鼻中的黏液含量增加。在这种哮喘模型中，允许剂量的氯暴露会增强 Th2 反应。功能改变与远端肺中 iNOS 和 ROCK-2 激活增加有关。
