白毛藤叶的抗真菌和细胞毒性活性。

Antifungal and cytotoxicity activities of Banisteriopsis argyrophylla leaves.

机构信息

Nucleus of Research in Natural Products (NuPPeN), Institute of Chemistry, Federal University of Uberlândia, Uberlândia, Brazil.

Trypanosomatids Laboratory (LATRI), Institute of Biomedical Sciences, Federal University of Uberlândia, Uberlândia, Brazil.

出版信息

J Pharm Pharmacol. 2018 Nov;70(11):1541-1552. doi: 10.1111/jphp.12996. Epub 2018 Aug 23.

DOI:10.1111/jphp.12996

PMID:30136729

Abstract

OBJECTIVES

This work aimed to evaluate the antifungal and cytotoxic activity of the EtOH extract and fractions of Banisteriopsis argyrophylla leaves, and to perform the identification of these bioactive metabolites.

METHODS

The EtOAc fraction (EAF) obtained from the ethanolic extract of B. argyrophylla leaves showed better antifungal potential against Candida spp. In this fraction, ten flavonoids have been identified by UHPLC-ESI-MS . Then, EAF was submitted to column chromatography to give four new fractions (A1-A4). The cytotoxicity was determined against Vero cells.

KEY FINDINGS

The EAF showed better antifungal potential against Candida spp. with minimum inhibitory concentrations (MICs) between 31.25 and 93.75 μg/ml. The (-)-catechin (fraction A1) showed a MIC of 2.83 μg/ml against Candida glabrata. Fractions A2, A3 and A4 were rich in quercetins and kaempferols and showed good inhibitory concentrations (5.86-46.87 μg/ml) against C. albicans, C. glabrata and C. tropicalis.

CONCLUSIONS

The EtOH extract, fractions and the isolated (-)-catechin showed lower toxicity to Vero cells than cisplatin, used as a positive control. Thus, the leaves of B. argyrophylla are a promising source of antifungal agents.

摘要

目的

本研究旨在评估蚬木叶片的乙醇提取物及其各馏分的抗真菌和细胞毒性活性，并对这些生物活性代谢物进行鉴定。

方法

从蚬木叶片的乙醇提取物中获得的乙酸乙酯馏分（EAF）对念珠菌属显示出更好的抗真菌潜力。在该馏分中，通过 UHPLC-ESI-MS 鉴定出了十种类黄酮。然后，EAF 被提交到柱色谱，得到四个新的馏分（A1-A4）。对 Vero 细胞进行细胞毒性测定。

主要发现

EAF 对念珠菌属具有更好的抗真菌潜力，最小抑菌浓度（MICs）在 31.25 和 93.75 μg/ml 之间。（-）-儿茶素（馏分 A1）对光滑念珠菌的 MIC 为 2.83 μg/ml。馏分 A2、A3 和 A4 富含槲皮素和山奈酚，对白色念珠菌、光滑念珠菌和热带念珠菌的抑制浓度（5.86-46.87 μg/ml）较好。