Schneider A, Schuhmann R, De Villiers E M, Knauf W, Gissmann L
Geburtshilfe Frauenheilkd. 1986 May;46(5):261-6. doi: 10.1055/s-2008-1035913.
Using filter in situ hybridisation for HPV-DNA detection we found among 217 women with positive cervical cytology a positive result in 152 cases (70%). The distribution of the different HPV types showed an association of HPV 6/11 mainly with benign lesions and of HPV 16/18 with obligatory precancer and invasive cervical cancer. In 2652 swabs of cytologically negative patients HPV-DNA was identified in 9.5%. The infection rate for HPV 16/18 in pregnant women was 6.4% against 2.3% in nonpregnant women. In a number of the patients with positive cervical cytology we additionally examined smears from the vagina and vestibulum for HPV-DNA: in 42% of the cases a positive HPV result was obtained in these areas as well. In about 50% of 39 male partners peniscopy revealed penile lesions and HPV-DNA was found in penile smears. A prospective cytological and virological study of cytologically positive patients showed a clear association of HPV 16/18 with progression of cervical lesions. In a cytologically negative group, follow-up examinations revealed HPV-DNA in 31%.
通过原位杂交法检测人乳头瘤病毒（HPV）-DNA，我们发现，在217例宫颈细胞学检查呈阳性的女性中，有152例（70%）检测结果呈阳性。不同HPV类型的分布情况显示，HPV 6/11主要与良性病变相关，而HPV 16/18则与癌前病变及浸润性宫颈癌密切相关。在2652例细胞学检查呈阴性的患者拭子样本中，9.5%检测出HPV-DNA。孕妇中HPV 16/18的感染率为6.4%，非孕妇为2.3%。对于一些宫颈细胞学检查呈阳性的患者，我们还对其阴道和前庭涂片进行了HPV-DNA检测：在42%的病例中，这些部位的检测结果也呈阳性。在39名男性伴侣中，约50%的阴茎检查发现阴茎有病变，阴茎涂片检测出HPV-DNA。一项针对细胞学检查呈阳性患者的前瞻性细胞学和病毒学研究表明，HPV 16/18与宫颈病变进展明显相关。在细胞学检查呈阴性的组中，随访检查发现31%的患者感染了HPV-DNA。
