Templeman Thomas S, DeMaggio Augustus E
Department of Biological Sciences, Dartmouth College, Hanover, New Hampshire, 03755.
Am J Bot. 1990 Feb;77(2):284-287. doi: 10.1002/j.1537-2197.1990.tb13553.x.
Onoclea sensibilis was found to contain globulin storage proteins of 2.0S and 11.3S. These globulins, comparable in size and subunit composition to the spore storage proteins characterized in Matteuccia struthiopteris (Templeman, DeMaggio, and Stetler, 1987), declined during imbibition and the initial stages of spore germination. Osmunda cinnamomea, a fern that is only distantly related to Matteuccia, also contained globulin proteins, but these had S values of 5.5 and 11.3. SDS-PAGE analysis revealed extensive differences in banding patterns of the 11.3S protein between Onoclea and Osmunda. This work indicates that while globulin proteins are important storage materials in a variety of ferns they exhibit considerable molecular heterogeneity. The observed heterogeneity in the globulin proteins may be a useful tool to explore evolutionary relationships in the ferns.
人们发现敏感鳞毛蕨含有2.0S和11.3S的球蛋白储存蛋白。这些球蛋白在大小和亚基组成上与荚果蕨中所鉴定的孢子储存蛋白相当（坦普尔曼、德马吉奥和斯特特勒，1987年），在吸胀和孢子萌发初期含量下降。桂皮紫萁是一种与荚果蕨亲缘关系较远的蕨类植物，也含有球蛋白，但这些球蛋白的S值为5.5和11.3。SDS - PAGE分析显示，鳞毛蕨和紫萁中11.3S蛋白的条带模式存在广泛差异。这项研究表明，虽然球蛋白是多种蕨类植物中的重要储存物质，但它们表现出相当大的分子异质性。在球蛋白中观察到的异质性可能是探索蕨类植物进化关系的有用工具。
